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一个完整的 MAP 激酶级联控制着形形成和 Verticillium dahliae 的毒性
Ziqin Ye1,2, Jun Qin3, Yu Wang1,2
1State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China.
aBIOTECH
|August 15, 2023
概括
在VdSte11-VdSte7-VdKss1基因激活蛋白激酶 (MAPK) 级联对于真菌病原体Verticillium dahliae形成hypopodia至关重要,使植物感染和致病性.
科学领域:
- 植物病理学 植物病理学
- 分子菌学分子菌学
- 生物化学 生物化学
背景情况:
- 植物病原体利用特殊的结构,如appressoria和haustoria透植物细胞.
- 土壤传播的真菌Verticillium dahliae形成hypopodia,分化为透钉用于感染.
- 在V. dahliae中调节hypopodium形成的分子机制尚不清楚.
研究的目的:
- 研究基激活蛋白激酶 (MAPKs) 在调节Verticillium dahliae.的hypopodium形成和致病性方面的作用.
- 为了确定涉及V. dahliae感染过程中的特定MAPK级联.
主要方法:
- 对于5个编码的MAPKs,V. dahliae的基因删除突变被创造出来.
- 突变物体的表型分析包括在体外海波形成和透测试中.
- 对棉花植物进行了致病性测定.
- 使用上游MAPK组件 (MAPKK VdSte7和MAPKK VdSte11) 的构成激活来评估它们对VdKss1激活的影响.
主要成果:
- 删除VdKss1,一个MAPK,显著损害hypopodium形成,体外透和棉花中的致病性.
- VdSte11-VdSte7-VdKss1路径被确定为一个核心的MAPK级联.
- 构成性激活VdSte7和VdSte11的特定激活VdKss1.1.
- 删除VdSte7或VdSte11导致类似于VdKss1删除突变的表型.
结论:
- 在VdSte11-VdSte7-VdKss1 MAPK级联中,对于调节V. dahliae中的低形成至关重要.
- 这种MAPK级联在V. dahliae对棉花植物的致病性中发挥着关键作用.
- 了解这种途径可以了解真菌与植物之间的相互作用,并为疾病控制提供潜在的目标.
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