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电比计光探针用于细胞器官中的pH映射

Subrata Munan¹, Rashmi Yadav¹, Niharika Pareek¹

¹Molecular Sensors and Therapeutics (MST) Research Laboratory, Department of Chemistry, School of Natural Sciences, Shiv Nadar Institute of Eminence Deemed to be University, NH 91, Tehsil Dadri, Uttar Pradesh, India 201314. animesh.samanta@snu.edu.in.

|August 15, 2023

概括

在有机体中精确地绘制细胞内pH值 (pHi),对于细胞平衡至关重要. 本次审查强调了用于器官 pH 监测的比度光探针的进展,解决了它们的发展方面的挑战.

科学领域:

细胞生物学细胞生物学
生物化学生物化学
分析化学分析化学

背景情况:

细胞内pH值 (pHi) 在各个器官细胞之间有所变化,维持它对于细胞平衡至关重要.
细胞器pHi的偏差会破坏细胞功能,包括增殖和离子运输,可能导致细胞死亡.
精确的有机体pH测绘对于了解细胞过程和疾病状态至关重要.

研究的目的:

审查用于器官特异性pH测绘的比例计光探针的近期进展.
讨论监测细胞内pH值变化 (ΔpHi) 在细胞器官中的重要性.
系统地阐述关于比度pH探针的开发策略.

主要方法:

对器官pH检测的比度光探针现有文献的综述.
对探头开发策略的分析,包括一个参考信号 (RFRS) 的比度光和两个可逆信号 (RFRvS) 的比度光.
讨论在细胞器官中映射ΔpHi的作用.

主要成果:

相对于pH监测的单色探针,相对度度光探针提供了自我校正的优势.
已报告了62个比度pH探针用于监测细胞内细胞器官的细胞内pH值变化.
缺乏专门针对细胞器特异性比度探针进行pH测绘的批判性审查.

结论:

电比测量光探头是精确的器官pH测绘的关键工具.
了解和开发这些探头对于推进细胞生物学和诊断至关重要.
需要进一步的研究来应对开发用于器官 pH 监测的先进比度探针所面临的挑战.

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