调查机会性肺性真菌共同感染的代谢和分子生态进化:基于实验室的横截面研究协议
Israel Kiiza Njovu1, Pauline Petra Nalumaga1, Lucas Ampaire2
1Medical Mycology Unit, Department of Microbiology, Faculty of Medicine, Mbarara University of Science and Technology, Mbarara, Uganda.
JMIR research protocols
|August 15, 2023
概括
这项研究调查了肺结核 (PTB) 患者的真菌和细菌共感染,揭示了关键的跨王国相互作用. 了解这些微生物动态是开发机会性肺性真菌共感染的有效治疗方法的关键.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 肺部病理学 肺部病理学
背景情况:
- 真菌-细菌共感染是肺结核 (PTB) 的新兴挑战.
- 在这些共感染中,致病机制和微生物群相互作用的理解很少.
- 研究微生物进化和宿主免疫逃避对于控制机会性肺性真菌共感染至关重要.
研究的目的:
- 研究机会性肺性真菌共感染的代谢和分子生态演变.
- 确定疑似PTB患者的负担,病因概况和微生物群落.
- 在肺共感染中描述真菌和细菌之间的相互作用.
主要方法:
- 基于实验室的横截面研究,共406名参与者.
- 使用常规和分子方法 (ITS和16S rDNA PCR) 对真菌和细菌病因分析唾液样本.
- 为肺微生物群落开发基因组规模的代谢模型,以分析微生物组合和宿主微生物相互作用.
主要成果:
- 该研究协议由姆巴拉拉大学研究伦理委员会和乌干达国家科学技术委员会批准.
- 患者招生于2022年3月开始;数据收集正在进行中,预计将于2023年8月完成.
- 详细说明发现的手稿将在完成后提交出版.
结论:
- 本协议将探讨假定PTB患者机会性肺性真菌共感染的代谢和分子生态演变.
- 建立关键的真菌-细菌跨王国协同作用关系对于理解和治疗共感染病因至关重要.
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