相关实验视频

Updated: Jul 19, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

序列逻辑和遗传电路的算法编程,用于记录益生菌细菌中的生物化学度

Matthew Lebovich^1,2, Min Zeng¹, Lauren B Andrews^1,2,3

¹Department of Chemical Engineering, University of Massachusetts Amherst, Amherst, Massachusetts 01003, United States.

ACS synthetic biology

|August 15, 2023

概括

具有遗传记忆电路的工程益生菌可以诊断肠道疾病. 本研究提出了一个计算方法来设计这些电路在益生菌大肠杆菌尼斯尔1917 (EcN) 准确的信号记录.

科学领域:

合成生物学合成生物学
微生物工程微生物工程
遗传电路设计的设计

背景情况:

工程益生菌通过报告检测到的生物标志物提供非侵入性胃肠道诊断.
遗传电路需要记忆功能来记录益生菌微生物中的信号.
了解临床相关菌株的电路组件性能,如埃舍里希亚大肠杆菌尼斯尔1917 (EcN) 是至关重要的.

研究的目的:

开发一种可扩展的计算方法,用于在Escherichia coli Nissle 1917 (EcN) 中设计强大的序列逻辑电路.
为了使工程益生菌能够记录和报告胃肠道内检测到的信号.
为肠道环境创建多重诊断工具.

主要方法:

利用TetR家族的转录抑制剂来构建序列逻辑电路的遗传逻辑门.
在实验中,在EcN中特征了16个遗传 NOT 门和9个传感器.
为遗传电路采用基于理论的,可扩展的计算设计方法.

主要成果:

在EcN中成功设计和预测了序列逻辑电路的性能.
证明了编码组合逻辑和顺序逻辑的遗传电路,其输出与预测相匹配.
开发了一种类似模拟的度记录电路,可以检测三种输入信号范围.

结论:

开发的计算方法使得在益生菌菌株EcN.中能够设计出强大的序列逻辑电路.
这项工作推动了工程益生菌作为具有遗传编码记忆的诊断工具的使用.
这些发现支持多重生物标志物查询和在肠道中记录模拟信号.

关键词:

工程设计的益生菌.遗传电路设计遗传电路设计基因编码的生物传感器生活的诊断生活的诊断非模型细菌是一种非模型细菌.合成基因调节合成基因调节全细胞生物传感器

更多相关视频

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

Immunometabolic Circuits in Infection for Advancing Host Directed Therapies

Immunometabolic Circuits in Infection for Advancing Host Directed Therapies

Published on: September 13, 2024

相关概念视频

Prokaryotic Transcriptional Activators and Repressors

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...

The Central Dogma

The Central Dogma

The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...

Bacterial Signaling

Bacterial Signaling

Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...

Coordination of Gene Expression Processes in Bacteria

Coordination of Gene Expression Processes in Bacteria

The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...

Global Regulatory Systems

Global Regulatory Systems

Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...

Operons

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by...