相关实验视频
Updated: Jul 19, 2025

08:56
Estrogen-Like Effect of Bazi Bushen Capsule in Ovariectomized Rats
Published on: April 7, 2023
936
骨细胞减少骨质保护素的表达可能有助于在停止使用denosumab后的反弹再吸收
Qiang Fu1,2, Nancy C Bustamante-Gomez1,2, Humberto Reyes-Pardo1,2
1Center for Musculoskeletal Disease Research.
JCI insight
|August 15, 2023
概括
停止服用德诺苏马布会导致由于反弹再吸收而导致骨质损失. 这项研究表明,骨质细胞减少生产骨质保护素 (OPG) 有助于这种效果.
科学领域:
- 骨生物学 骨生物学
- 内分泌学 在内分泌学.
- 药理学 药理学是指药理学的学科.
背景情况:
- 丹诺苏马布是一种抗RANKL抗体,有效降低骨再吸收和骨折风险.
- 突如其来的停止denosumab治疗导致快速的骨质损失和增加的吸收,不清楚的潜在分子机制.
研究的目的:
- 为了研究细胞和分子机制驱动的反弹骨吸收后停止使用denosumab.
- 在一个人性化的RANKL小鼠模型中,检查denosumab对骨细胞功能和骨质保护素 (OPG) 表达的影响.
主要方法:
- 为了进行德诺苏马布治疗研究,生成了人性化的RANKL小鼠.
- 评估了骨质细胞和骨质母细胞在无骨骨中的数量.
- 在骨髓中的量化骨质细胞原始体.
- 在骨组织中测量了骨质保护素 (OPG) mRNA水平.
- 在骨细胞,特别是骨细胞内局部化OPGmRNA表达.
主要成果:
- 德诺苏马布显著抑制了骨质细胞和骨质细胞的数量.
- 骨质细胞原体数量保持不变,表明抑制发生在原体后阶段.
- 长期的德诺苏马布治疗减少了骨质细胞OPG mRNA的表达.
- 骨细胞OPG表达局限于靠近骨表面的细胞,这表明与年龄相关或与表面相关的调节.
结论:
- 在denosumab治疗期间减少新骨细胞的形成可能会降低OPG的产生.
- 这种OPG的下降,骨再吸收的关键抑制剂,可能有助于在停止使用denosumab后观察到的反弹效应.
- 骨细胞衍生的OPG在调节骨重塑和减轻再吸收方面发挥着至关重要的作用.
相关概念视频
Osteoclasts in Bone Remodeling
3.0K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
3.0K
Bone Remodeling
38.4K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
38.4K
Bone Disorders
3.7K
Aging and its effect on bone remodeling is the most common cause of bone disorders. In young and healthy people, bone deposition and resorption happen at an equal rate to maintain optimal bone health.
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
3.7K
Hormones and Bone Tissue
2.7K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
2.7K
Skeleton and Calcium Homeostasis
4.6K
Calcium is not only the most abundant mineral in bone but also the most abundant mineral in the human body. Calcium ions are needed for bone mineralization, tooth health, heart rate regulation and strength of contraction, blood coagulation, the contraction of smooth and skeletal muscle cells, and the regulation of nerve impulse conduction. The average calcium level in the blood is about 10 mg/dL. When the body cannot maintain this level, a person will experience hypo or hypercalcemia.
4.6K
GPCR Desensitization
6.1K
G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
6.1K

