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全基因组的CRISPR屏幕识别非正规翻译因子eIF2A作为SARS-CoV-2编程-1核糖体框架转移的增强剂

Lian-Huan Wei¹, Yu Sun¹, Junjie U Guo¹

¹Department of Neuroscience, Yale University School of Medicine, New Haven, CT 06520, USA.

|August 15, 2023

概括

这项研究确定了真核转化启动因子2A (eIF2A) 作为一种增强SARS-CoV-2编程-1核糖体框架转移的宿主因子. 失去eIF2A减少了病毒复制,突出显示了它在冠状病毒转化中的作用.

科学领域:

分子生物学分子生物学
病毒学病毒学
遗传学遗传学是一个

背景情况:

阳性链RNA病毒,包括冠状病毒,利用编程的-1核糖体框架转移 (-1PRF) 来进行多基RNA转化.
调节病毒-1PRF的宿主因素在很大程度上是未知的.

研究的目的:

使用全基因组屏幕识别调节SARS-CoV-2 -1 PRF的宿主因素.
调查已识别的因素在病毒复制和翻译中的特定作用.

主要方法:

全基因组的CRISPR-Cas9淘汰屏幕用于识别宿主因素.
试验评估宿主因素对SARS-CoV-2的直接影响 -1 PRF.
测量具有改变宿主因子水平的细胞中SARS-CoV-2复制的结果.
全转录组分析以确定RNA结合偏好.

主要成果:

识别了抑制或增强SARS-CoV-2的宿主因素.
发现真核细胞翻译启动因子2A (eIF2A) 能够独立于翻译启动,特别增强-1 PRF.
失去eIF2A显著减少了SARS-CoV-2的复制.
eIF2A优先结合富含CG的RNA基因,包括靠近SARS-CoV-2框架转移刺激元件 (FSE) 和核糖体的区域.

结论:

eIF2A在增强SARS-CoV-2 -1 PRF中发挥着至关重要的作用.
eIF2A调节病毒RNA翻译独立于其在启动中的正规作用.
准eIF2A可能是抑制SARS-CoV-2复制的潜在策略.

关键词:

CP: 微生物学微生物学这是一个CRISPR屏幕.这就是SARS-CoV-2病毒.eIF2A 如果有编程 −1 核糖体框架转移.翻译翻译翻译翻译翻译翻译

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