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相关概念视频

Sanger Sequencing01:57

Sanger Sequencing

754.8K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
754.8K
Next-generation Sequencing03:00

Next-generation Sequencing

91.5K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
91.5K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

18.9K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.9K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.2K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.2K

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相关实验视频

Updated: Jul 19, 2025

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

12.4K

改进了基因大小DNA构造的组合组合和条形码测序.

Diana Hernandez Hernandez1, Lin Ding1, Ayako Murao1

  • 1Synthetic Biology and Bioenergy Group, J. Craig Venter Institute, La Jolla, California 92037, United States.

ACS synthetic biology
|August 15, 2023
PubMed
概括

工程生物学使用DNA条形编码来分析复杂的基因相互作用,克服组合挑战. 这种方法提高了生物系统的性能,并有助于发现各种应用的基因协同作用.

关键词:
大众组合遗传学通过核酶辅助的酶性结合.史诗主义就是一种史诗主义.基因相互作用 基因相互作用多基因协同作用,用于生物封闭.这是下一代测序.

更多相关视频

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

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Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly
10:18

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly

Published on: February 9, 2015

37.2K

相关实验视频

Last Updated: Jul 19, 2025

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

12.4K
Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

13.6K
Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly
10:18

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly

Published on: February 9, 2015

37.2K

科学领域:

  • 合成生物学 合成生物学
  • 分子生物学分子生物学
  • 基因组学就是基因组学.

背景情况:

  • 协同基因相互作用对于提高生物系统性能至关重要.
  • 分析多基因相互作用是由于组合复杂性的计算挑战.
  • 基因条形编码和下一代测序为分析基因相互作用提供了潜在的解决方案.

研究的目的:

  • 改进CombiGEM方法,以便在合成生物学中得到更广泛的应用.
  • 为了使典型的基因大小的DNA片段的组装.
  • 为了降低小规模基因工程项目的测序成本.

主要方法:

  • 改进了用于DNA组装的CombiGEM方法.
  • 整合用于基因识别的DNA条形码.
  • 应用下一代测序来分析组合图书馆.

主要成果:

  • 改进的CombiGEM方法成功地组装了典型的基因大小的DNA片段.
  • 较低的测序成本使该方法在小规模项目中更容易使用.
  • 扩大了超越小RNA基因的适用性到更大的基因结构.

结论:

  • 改进的CombiGEM方法有助于研究生物系统中的基因协同作用.
  • 这种方法支持在自然和人工过程中发现基因相互作用.
  • 应用包括生物封闭,化合物生产和理解基本的生物机制.