Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Preparation of Optical-Based Sensors for the Determination of Cardiac Myosin-Binding Protein C.

ACS omega·2026
Same author

Molecularly imprinted polymers: Recent advances in protein chromatography.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026
Same author

Immunoaffinity biosensors for deoxynivalenol determination from wheat sample: the evaluation of antibody immobilization route and surface topography on the sensor performance.

Mikrochimica acta·2026
Same author

Ultrasensitive Human Urinary Albumin Detection via Composite Nanohydrogels.

Micromachines·2026
Same author

Clinical outcomes of parathyroidectomy in primary hyperparathyroidism: a 10-year single-center experience with 115 procedures.

European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·2026
Same author

Comparison of EMG responses obtained from endotracheal tube and thyroid cartilage surface electrodes in thyroidectomies performed with intraoperative nerve monitoring.

European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·2026

相关实验视频

Updated: Jul 19, 2025

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening
11:28

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

Published on: October 4, 2017

10.3K

基于分子印记的微凝用于血栓净化.

Merve Asena Özbek1, Erdoğan Özgür2, Nilay Bereli2

  • 1Department of Chemistry Division, Institute of Science, Hacettepe University, Ankara, Turkey; Hacettepe University, Faculty of Science, Department of Chemistry, Ankara, Turkey.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
|August 15, 2023
PubMed
概括

研究人员开发了可重复使用的选择性微凝,用于高效的血栓净化. 这种低成本的方法增强了蛋白质净化和酶生产,这对于血液静止研究至关重要.

关键词:
亲和色谱法是一种亲和色谱法.微凝是微凝中的一种.分子印记是一种分子印记.蛋白质净化 蛋白质的净化血栓激素 血栓激素 是一种

更多相关视频

A Microfluidic Approach for the Study of Ice and Clathrate Hydrate Crystallization
08:01

A Microfluidic Approach for the Study of Ice and Clathrate Hydrate Crystallization

Published on: August 18, 2022

3.1K
Protein Purification-free Method of Binding Affinity Determination by Microscale Thermophoresis
10:22

Protein Purification-free Method of Binding Affinity Determination by Microscale Thermophoresis

Published on: August 15, 2013

30.6K

相关实验视频

Last Updated: Jul 19, 2025

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening
11:28

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

Published on: October 4, 2017

10.3K
A Microfluidic Approach for the Study of Ice and Clathrate Hydrate Crystallization
08:01

A Microfluidic Approach for the Study of Ice and Clathrate Hydrate Crystallization

Published on: August 18, 2022

3.1K
Protein Purification-free Method of Binding Affinity Determination by Microscale Thermophoresis
10:22

Protein Purification-free Method of Binding Affinity Determination by Microscale Thermophoresis

Published on: August 15, 2013

30.6K

科学领域:

  • 生物化学 生物化学
  • 材料科学 材料科学 材料科学

背景情况:

  • 高效的蛋白质净化对于研究和酶生产至关重要.
  • 凝血素在静血过程中起着至关重要的作用.
  • 现有的净化方法可能昂贵且低效.

研究的目的:

  • 开发一种新的,具有成本效益的,可重复使用的血栓净化方法.
  • 为了创建高度选择性的血栓印记微型晶.

主要方法:

  • 使用包括微计算机断层扫描 (μCT),扫描电子显微镜 (SEM) 和BET表面积分析在内的技术合成和特征化微晶.
  • 评估了血吸附能力和选择性.
  • 使用快速性能液体染色学 (FPLC) 评估了可重复使用性和纯度.

主要成果:

  • 开发的微凝表现出高的血栓吸附能力 (Qmax: 55.86 mg/g).
  • 吸附动力学遵循伪秒序模型,平衡数据符合兰格穆尔等温模型.
  • 微凝显示出良好的选择性和可重复使用性,血栓纯度通过FPLC得到证实.

结论:

  • 血栓印记微凝为血栓净化提供了一个有希望的,高效的,可重复使用的解决方案.
  • 这种方法在生物化学研究和酶生产中具有很大的应用潜力.
  • 开发的材料提供了对传统净化技术的选择性和经济有效的替代方案.