在上胃肠道研究中的采样方法
Hyo-Joon Yang1, Seung In Seo2, Jin Lee3
1Division of Gastroenterology, Department of Internal Medicine and Gastrointestinal Cancer Center, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea.
本综述提出了用于上部胃肠道研究的内镜活检方案. 它详细介绍了微生物组,DNA,RNA,蛋白质,单细胞RNA测序和有机体培养的最佳样本采集.
科学领域:
- 胃肠病学 胃肠病学
- 翻译研究是翻译研究.
- 活检技术 活检技术
背景情况:
- 在上部胃肠道 (GI) 研究中的翻译研究已经取得了显著的进展.
- 内镜活检是一种低风险的方法,用于获得上部肠道组织,即使在不可切割的胃癌中也适用.
- 缺乏用于内镜活检样本采集和储存的标准化协议,但对于研究完整性至关重要.
研究的目的:
- 提出一个全面的协议,从内镜活检收集上部胃肠道组织样本.
- 为研究人员提供最佳样本采集指南,用于各种分析,包括微生物组,分子和有机体研究.
- 解决对上部肠道内镜活检研究中标准化方法的需求.
主要方法:
- 进行了全面的文献审查,以确定内镜活检样本采集的现有方法和最佳实践.
- 该综述综合了对各种研究应用的样本要求的信息.
- 根据文献综述的发现,制定了一项协议.
主要成果:
- 对于微生物组分析,建议使用1-2个活检组织,5毫升胃液和3-4毫升唾液.
- DNA/RNA分析至少需要一个活检组织;蛋白质组学需要2-3个组织.
- 单细胞RNA测序需要3-5个组织,首选额外的样本;有机体培养需要多个样本以获得质量.
结论:
- 对于可靠的上部胃肠道研究,内镜活检样本采集的标准化协议至关重要.
- 对于微生物组,分子分析和有机体培养,建议使用特定的样本数量.
- 本议定书旨在提高上部胃肠道领域的翻译研究的质量和可重复性.
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