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相关概念视频

Sampling Methods: Sample Types01:18

Sampling Methods: Sample Types

274
Sampling materials are classified into three main types: solid, liquid, and gas.
Solid samples include a variety of substances, such as sediments from water bodies, soil, metals, and biological tissues. Two standard methods for extracting sediments from water bodies are grab sampling and piston coring. Grab sampling involves using a device to collect a discrete sediment sample from the bottom of a water body with minimal disturbance. Grab samples do not always represent the entire area due to...
274
Upper GI Series: Barium Swallow01:24

Upper GI Series: Barium Swallow

484
The Barium Swallow Study, or a Barium Esophagogram, is a diagnostic imaging method used to visualize the upper gastrointestinal (GI) tract, including the esophagus, stomach, and small intestine. It employs barium sulfate, a radiopaque contrast material, to provide clear images of the upper digestive system, helping to identify abnormalities, diseases, or structural issues.
Purpose and Procedure
Patients undergoing this procedure ingest a liquid containing barium sulfate with a chalky...
484
Assessment of the Gastrointestinal System I: Subjective Data01:17

Assessment of the Gastrointestinal System I: Subjective Data

225
Assessing the gastrointestinal (GI) system is a complex process that begins with collecting subjective data. This data, collected through patient interviews, provides crucial insights into the patient's health history, perception patterns, and lifestyle habits, all contributing significantly to GI health.
Health History
The initial step in assessing the GI system is obtaining a comprehensive health history. This includes inquiring about the patient's history or presence of problems...
225
Peptic Ulcer Disease III: Clinical Manifestations and Diagnostic Studies01:28

Peptic Ulcer Disease III: Clinical Manifestations and Diagnostic Studies

157
Peptic ulcer disease (PUD) presents with diverse symptoms depending on the location and severity of the ulcer. Clinical manifestations of peptic ulcer include dull pain and a burning sensation in the mid-epigastric region.
Few clinical manifestations differentiate gastric ulcers from duodenal ulcers. Distinctions in the location, timing, and pain relief are crucial for healthcare providers in differentiating between gastric and duodenal ulcers during clinical assessments.
157
Methods for Studying Drug Absorption: In situ01:09

Methods for Studying Drug Absorption: In situ

266
In situ experiments, such as the Doluisio method and Single-Pass Perfusion technique, provide critical insights into drug uptake by simulating in vivo conditions for drug absorption.
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
266
Endoscopic Procedures I: Esophagogastroduodenoscopy01:29

Endoscopic Procedures I: Esophagogastroduodenoscopy

176
An Esophagogastroduodenoscopy (EGD) is a diagnostic procedure in which an endoscopist uses a flexible, lighted endoscope to visualize the upper gastrointestinal (GI) tract. The procedure includes visualizing the oropharynx, esophagus, stomach, and the first part of the small intestine, the duodenum.
During an EGD, the endoscope can be used to:
176

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相关实验视频

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Gastric Mucosa Quantitative Polymerase Chain Reaction Analysis for Detecting Helicobacter pylori and Antibiotic Resistance
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在上胃肠道研究中的采样方法.

Hyo-Joon Yang1, Seung In Seo2, Jin Lee3

  • 1Division of Gastroenterology, Department of Internal Medicine and Gastrointestinal Cancer Center, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea.

Journal of Korean medical science
|August 15, 2023
PubMed
概括
此摘要是机器生成的。

本综述提出了用于上部胃肠道研究的内镜活检方案. 它详细介绍了微生物组,DNA,RNA,蛋白质,单细胞RNA测序和有机体培养的最佳样本采集.

关键词:
活检是为了检查生物质.内镜检查是指内镜检查.样本 样本 样本 样本 样本翻译研究是翻译研究.

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科学领域:

  • 胃肠病学 胃肠病学
  • 翻译研究是翻译研究.
  • 活检技术 活检技术

背景情况:

  • 在上部胃肠道 (GI) 研究中的翻译研究已经取得了显著的进展.
  • 内镜活检是一种低风险的方法,用于获得上部肠道组织,即使在不可切割的胃癌中也适用.
  • 缺乏用于内镜活检样本采集和储存的标准化协议,但对于研究完整性至关重要.

研究的目的:

  • 提出一个全面的协议,从内镜活检收集上部胃肠道组织样本.
  • 为研究人员提供最佳样本采集指南,用于各种分析,包括微生物组,分子和有机体研究.
  • 解决对上部肠道内镜活检研究中标准化方法的需求.

主要方法:

  • 进行了全面的文献审查,以确定内镜活检样本采集的现有方法和最佳实践.
  • 该综述综合了对各种研究应用的样本要求的信息.
  • 根据文献综述的发现,制定了一项协议.

主要成果:

  • 对于微生物组分析,建议使用1-2个活检组织,5毫升胃液和3-4毫升唾液.
  • DNA/RNA分析至少需要一个活检组织;蛋白质组学需要2-3个组织.
  • 单细胞RNA测序需要3-5个组织,首选额外的样本;有机体培养需要多个样本以获得质量.

结论:

  • 对于可靠的上部胃肠道研究,内镜活检样本采集的标准化协议至关重要.
  • 对于微生物组,分子分析和有机体培养,建议使用特定的样本数量.
  • 本议定书旨在提高上部胃肠道领域的翻译研究的质量和可重复性.