减光谱作为六种分辨率技术的补充方法来解决重叠的光谱;应用到具有绿色和白色评估的多组分兽医配方
Mahmoud G Hagag1, Ahmed M Hemdan1, Ahmed H Nadim2
1Analytical Chemistry Department, Faculty of Pharmacy, Ahram Canadian University, 6 October City, 4th Industrial Zone, Banks Court Street, Giza, Egypt.
BMC chemistry
|August 15, 2023
概括
6种光谱法用于分析三班达 (TCB) 和levamisole HCl (LVM) 混合物. 该研究强调了用于兽药药物分析的经济,准确和绿色分析化学方法.
科学领域:
- 分析化学 分析化学
- 频谱光度测量是一种光谱光度测量.
- 绿色分析化学 绿色分析化学
背景情况:
- 在二元混合物中重叠的光谱带来了分析挑战.
- 光谱光度法为制药化合物提供经济和精确的分析.
研究的目的:
- 为了比较六种经济,准确和精确的光谱测量方法,用于Triclabendazole (TCB) 和Levamisole HCl (LVM) 的确定.
- 评估数学过技术在零和第一阶光谱解决二进制混合物.
- 评估开发的分析方法的绿色性.
主要方法:
- 对六种光谱光度法进行比较分析:比率减法,常数乘法,因子反应和光谱减法以零和第一导数顺序.
- 确定TCB和LVM的线性范围,检测极限 (LOD).
- 根据VICH指南进行验证.
- 使用NEMI,AES,GAPI和AGREE工具进行绿色评估,以及RGB模型白色评估.
主要成果:
- 所有提出的方法都显示出高准确度和精度,与报告的技术相比.
- 对TCB (1.0-10.0μg/mL或2.0-20.0μg/mL) 和LVM (2.0-14.0μg/mL) 建立了线性范围.
- TCB的LOD值为0.08微克/毫升,LVM的LOD值为0.19微克/毫升.
- 开发的方法与绿色分析化学原则保持一致.
结论:
- 该研究使用各种数学过技术成功地在二进制混合物中分解了TCB和LVM.
- 拟议的光谱光学方法是散装材料和兽医配方常规分析的经济,准确和精确的替代方案.
- 绿色评估证实了开发的分析程序的环境可行性.
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