相关实验视频

Updated: Jul 19, 2025

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

缺氧MCF7和PANC-1细胞的miRNA差异表达

Sandy M Al-Sisan¹, Malek A Zihlif¹, Hana M Hammad²

¹Department of Pharmacology, School of Medicine, The University of Jordan, Amman, Jordan.

Frontiers in endocrinology

|August 16, 2023

概括

这项研究表明,慢性循环缺氧通过改变microRNA (miRNA) 表达显著影响癌症的进展,导致胰腺和乳腺癌细胞的化学抵抗. 特定的hypoxamiRs被确定为这些变化的关键驱动因素,提供潜在的治疗点.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
癌症研究癌症研究

背景情况:

缺氧是瘤微环境中的一个关键因素,它影响了诸如增殖,新陈代谢,亡,DNA修复和化学抵抗等关键细胞过程.
缺氧诱导微RNA (miRNA) 表达的显著变化,称为hypoxamiRs,在癌症进展中起着至关重要的作用.

研究的目的:

调查 hypoxamiRs 在胰腺癌和乳腺癌进展中的作用.
通过了解特定的低氧化物对癌症发展的贡献来确定新的治疗点.

主要方法:

乳腺癌MCF7细胞和胰腺癌PANC-1细胞在20个周期内暴露于慢性循环缺氧 (72小时缺氧/24小时补氧).
在10和20个循环后,使用RT-PCR阵列进行miRNA表达概况分析.
使用MTT试验评估了细胞增殖和对多克索鲁比的化学抵抗.

主要成果:

慢性循环性缺氧诱导了EMT类表型和两个细胞系中的多克索鲁比辛耐药性.
缺氧的PANC-1细胞保持了相似的翻倍时间,而缺氧的MCF7细胞显著增加了翻倍时间.
特定的miRNA被确定为丰富胰腺和乳腺癌在缺氧下进展的特征至关重要.

结论:

特定的hypoxamiRs (例如,PANC-1的miR-221,miR-21,miR-155,miR-34;MCF7的miR-93,miR-20a,miR-15,miR-17) 与癌症特征,EMT,增殖变化和化学抵抗有关.
慢性循环缺氧对于胰腺癌和乳腺癌细胞中产生耐化学反应的表型至关重要.
需要进一步的研究,以阐明这些miRNAs影响癌症进展和化学抵抗的确切机制.

关键词:

乳腺癌乳腺癌乳腺癌慢性循环性缺氧慢性循环性缺氧循环林依赖性激酶是一种依赖性激酶.多克索鲁比的化学抵抗性表皮细胞到介质细胞的过渡.这种药物是hypoxamirs.这是一个小RNARNA.胰腺管道腺癌瘤

更多相关视频

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...