氨酸通过控制SIRT1活动来调节有机细胞的分布和功能,这是在排卵后卵细胞衰老过程中
Xupeng Xing1,2, Jingfeng Peng1,2, Jingyu Zhao3,4
1National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, China.
Frontiers in nutrition
|August 16, 2023
概括
素是一种SIRT1激活剂,可以恢复老化卵细胞中的细胞器功能. 这种天然化合物通过调节线粒体,内分泌网膜和戈尔吉器官的分布和活动来保护卵细胞质量.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 卵细胞质量对发育能力至关重要,如果受精延迟,在排卵后迅速下降.
- 已知Sirtuin 1 (SIRT1) 在排卵后衰老期间保护卵细胞质量.
- 在老化卵细胞中,SIRT1对有机细胞功能 (线粒体,ER,戈尔吉细胞,溶解体) 的特定影响仍然在很大程度上未被研究.
研究的目的:
- 为了研究在排卵后老化的卵细胞中关键细胞器的分布和功能.
- 确定白素激活SIRT1在缓解与年龄相关的卵细胞功能障碍中的作用.
- 阐明白素影响器官完整性和功能的机制.
主要方法:
- 对老化卵细胞与年轻卵细胞中器官分布和蛋白质表达的比较分析.
- 用黄素 (SIRT1激活剂) 和EX-527 (SIRT1抑制剂) 治疗老化的卵细胞.
- 评估线粒体膜潜力,ER压力标志物 (GRP78),溶酶体标志物 (LAMP1) 和戈尔吉复合蛋白 (GOLPH3) 的评估.
主要成果:
- 排卵后卵细胞衰老导致线粒体,ER和戈尔吉器官的异常分布.
- 素治疗恢复了正常的器官分布,并纠正了改变的蛋白质水平 (GRP78,LAMP1,GOLPH3) 和线粒体膜潜力.
- 用EX-527抑制SIRT1逆转了黄素的保护作用,证实了SIRT1的关键作用.
结论:
- 通过激活SIRT1,氨酸在排卵后衰老过程中有效地保持卵细胞质量.
- 在老化卵细胞中,SIRT1激活对于维护线粒体,ER,戈尔吉器官和溶酶体的适当分布和功能至关重要.
- 这些发现突出显示黄素是改善卵细胞质量和生育结果的潜在治疗剂.
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