相关实验视频

Updated: Jul 19, 2025

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

自动LC-MS/MS:准备好临床常规实验室工作吗?

Sina Junger¹, Miriam Hoene², Maria Shipkova¹

¹SYNLAB MVZ Leinfelden-Echterdingen GmbH, Leinfelden-Echterdingen, Germany.

Journal of mass spectrometry and advances in the clinical lab

|August 16, 2023

概括

一个完全自动化的液体染色学-并联质谱学 (LC-MS/MS) 系统证明了维生素D和免疫抑制药物测试的高精度和准确性. 这种自动化LC-MS/MS适用于商业实验室的常规诊断.

科学领域:

临床化学临床化学
分析化学分析化学
质谱测量质量谱测量

背景情况:

液体染色学-并联质谱学 (LC-MS/MS) 为临床诊断提供了高灵敏度和特异性.
常规临床实验室使用LC-MS/MS受到复杂性和缺乏自动化的限制.
之前的研究表明,在医院环境中,自动化LC-MS/MS的性能很好.

研究的目的:

评估完全自动化的LC-MS/MS系统在商业实验室环境中的适用性.
评估各种实验室操作员对自动化LC-MS/MS系统的可用性.
将自动化系统的性能与商业实验室现有的方法进行比较.

主要方法:

自动LC-MS/MS的分析性能在5个月内对25-氧维生素D和免疫抑制药物 (ISD) 进行了评估.
测试结果与医院实验室的相同分析仪,内部LC-MS/MS方法和化学发光微粒免疫测试 (CMIA) 相比较.
九个运营商通过结构化问卷评估了系统的可用性.

主要成果:

自动化系统表现出高精度 (CV<8%) 和准确性 (偏差<7%),与外部质量评估样本保持良好一致.
结果与医院实验室中相同的分析仪相当.
与内部LC-MS/MS方法相比,对25-OH维生素D3,环素A和塔克罗利斯观察到可接受的中位偏差;25-OH维生素D3与免疫试验的偏差是可以接受的.
所有运营商都报告了积极的用户体验.

结论:

一个完全自动化的LC-MS/MS系统非常适合在商业实验室中整合到常规诊断中.
该系统的可用性和分析性能支持其在各种实验室环境中采用.
自动化解决了复杂性,并提高了LC-MS/MS在临床诊断中的常规应用.

关键词:

自动化自动化自动化形离子分析仪形离子分析仪环素A 是一种环素.这是LC-MS/MS.塔克罗利慕斯可以使用.维生素D 维生素D是一种维生素.

更多相关视频

$Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems$ 14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

相关概念视频

Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

Tandem Mass Spectrometry

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...