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在普通小麦中对多氧化酶1基因的功能分析

Shengnan Zhai¹, Hang Liu², Xianchun Xia³

¹National Engineering Laboratory for Wheat and Maize, Key Laboratory of Wheat Biology and Genetic Improvement in the Northern Yellow-Huai Rivers Valley of Ministry of Agriculture and Rural Affairs, Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, China.

Frontiers in plant science

|August 16, 2023

概括

在小麦中,多氧化酶 (PPO) 的活性会导致色. 研究人员使用RNA干扰和TILLING研究Ppo1基因,识别了PPO活性降低的突变物,用于开发低色小麦品种.

科学领域:

植物分子生物学植物分子生物学
农业科学农业科学
生物化学生物化学

背景情况:

多氧化酶 (PPO) 活性是小麦制品不良色的关键因素.
已经确定了PPO活动的主要贡献者Ppo1基因,但其精确的功能和调节机制需要进一步阐明.

研究的目的:

研究小麦Ppo1基因的功能和遗传调节.
为潜在的育种应用确定小麦突变物具有降低的PPO活性.

主要方法:

用RNA干扰 (RNAi) 来降低转基因小麦系中的Ppo1转录水平.
在基因组中向诱导局部病变 (TILLING) 技术被用于选乙基甲硫酸盐 (EMS) 变异的人群中的Ppo1突变物.

主要成果:

RNAi线路显示Ppo1转录水平 (15.5%-60.9%) 和PPO活性 (12.9%-20.4%) 显著下降.
确定了32种Ppo1突变,其中两种误解突变 (M092141和M091098) 显示Ppo1表达 (6.7%-54.4%) 和PPO活性 (28.8%-29.7%) 降低.
序列分析表明,M092141和M091098的突变部位高度保存,位于活跃部位附近,这表明它在PPO1功能中起着关键作用.

结论:

Ppo1基因在调节小麦中PPO活性方面发挥着至关重要的作用.
已识别的M092141和M091098突变代表了开发低谷物PPO活性小麦品种的宝贵遗传资源.
这项研究提供了对Ppo1分子机制的见解,并提供了改进小麦PPO活性遗传的策略.

关键词:

公共事业组织活动活动PPO活动它们是RNAiRNAi.计时计时计时计时计时基因功能基因功能基因功能遗传调节是一种基因调节.

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