[血管酶转化酶2通过抑制IL-6/JAK2/STAT3信号通路来缓解传染性支气管炎病毒诱导的细胞炎症]
Xiaoxia Ji1, Huanhuan Wang1, Chang Ma1
1Key Laboratory of Animal Physiology and Biochemistry, Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
ангиотензин转化酶2 (ACE2) 缓解由禽传染性支气管炎病毒 (IBV) 引起的细胞炎症. ACE2 阻止IL-6/JAK2/STAT3通路的激活,减少Vero和DF-1细胞中IBV诱导的炎症.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 鸟类传染性支气管炎病毒 (IBV) 导致显著的细胞炎症.
- 血管酶转化酶2 (ACE2) 在调节IBV诱导的炎症中的作用尚未完全理解.
研究的目的:
- 研究ACE2对IBV诱导的细胞炎症的调节作用.
- 阐明涉及IL-6/JAK2/STAT3信号通路的潜在分子机制.
主要方法:
- 使用了暴露于IBV的Vero和DF-1细胞.
- 通过过度表达和耗尽 (siRNA) 操纵ACE2水平.
- 通过使用RT-qPCR,ELISA和西方斑点测试,评估病毒复制 (IBV-N mRNA),炎症标记物 (IL-6) 和信号通路激活 (JAK2 / STAT3酸化).
主要成果:
- 过度表达ACE2可以缓解IBV诱导的细胞病变效应和炎症.
- ACE2 枯竭加剧了这些影响.
- 感染IBV增加了IL-6和JAK2/STAT3的酸化,而ACE2的表达减少.
- 过度表达ACE2降低了IL-6和JAK2/STAT3的酸化,但没有影响IBV复制.
- ACE2 枯竭增加了IL-6,但没有显著改变JAK2/STAT3酸化.
结论:
- ACE2对IBV诱导的细胞炎症起着保护作用.
- 通过抑制IL-6/JAK2/STAT3信号通路,ACE2可以减轻炎症.
- ACE2不会影响DF-1细胞中的IBV复制,但会调节宿主炎症反应.
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