相关实验视频

Updated: Jul 19, 2025

In Vitro Assay to Study Tumor-macrophage Interaction

In Vitro Assay to Study Tumor-macrophage Interaction

Published on: August 1, 2019

免疫刺激剂水凝导向瘤微环境重编程以有效地增强巨介导细胞化作用,用于全身癌症免疫疗法

Jun-Long Liang¹, Xiao-Kang Jin¹, Guo-Feng Luo²

¹Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, P. R. China.

|August 16, 2023

概括

这项研究设计了一种水凝,以增强巨细胞介导细胞化 (MMCP) 用于癌症免疫治疗. 水凝有效地准瘤细胞,并重塑瘤微环境 (TME),以提高抗瘤免疫力.

科学领域:

免疫学免疫学免疫学
生物材料科学生物材料科学
纳米技术纳米技术

背景情况:

巨细胞媒介细胞化 (MMCP) 对于抗瘤免疫疗法至关重要.
瘤细胞避开了细胞分裂,瘤微环境 (TME) 是免疫抑制的,阻碍了MMCP.
开发克服这些障碍的策略对于有效的癌症免疫疗法至关重要.

研究的目的:

设计一种能够促进MMCP的新型水凝 (TCCaGM).
研究水凝调节TME并增强抗瘤免疫反应的能力.
评估水凝与PD-1抗体的联合治疗,以改善癌症免疫疗法.

主要方法:

通过使用CaCO3纳米粒子,设计了一种水凝 (TCCaGM) 封装颗粒细胞 - 巨细胞殖民地刺激因子 (GM-CSF) 和一个纳米平台 (TCCaN) 带有突尼胺素 (Tuni) 和催化酶 (CAT).
评估了水凝对缺氧/酸性TME的影响,对"吃我"信号 (calreticulin,CRT) 的上调和对瘤细胞"不要吃我"信号 (CD47) 的下调.
评估了树突细胞 (DCs) 的招募和激活,瘤相关巨细胞 (TAMs) 向M1-类表型的极化,以及CD8+ T细胞的反应.
研究了水凝与PD-1抗体 (αPD-1) 对全身抗瘤免疫,瘤控制,转移和复发的协同作用.

主要成果:

工程水凝 (TCCaGM) 有效地缓解了缺氧/酸性TME.
观察到CRT的上调和CD47的下调在瘤细胞上,促进细胞化.
水凝增强了DC招募/激活,并促进了TAMs向抗瘤M1表型的两极分化.
与αPD-1的联合治疗显著增强了全身抗瘤免疫力,控制了瘤生长,并抑制了转移和复发.

结论:

TCCaGM水凝是一种增强MMCP和克服TME诱导的免疫抑制的有希望的策略.
工程水凝有效地重新编程TME以促进抗瘤免疫反应.
与αPD-1抗体的组合疗法为具有长期效果的高性能抗瘤免疫疗法提供了一种强有力的方法.

关键词:

CD47-SIRPα 相互作用抗瘤免疫疗法免疫疗法巨细胞的 fagocytosis 的发生.热敏水凝是一种热敏水凝.瘤微环境是一个微环境.

更多相关视频

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Published on: November 28, 2019

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion

Published on: October 20, 2016

相关概念视频

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.