在大脑麻的子模型中,脊柱运动神经元对血清素有异常反应
Emily J Reedich1,2, Landon T Genry1,2,3, Preston R Steele1,2,3
1George and Anne Ryan Institute for Neuroscience, University of Rhode Island, Kingston, Rhode Island, USA.
The Journal of physiology
|August 16, 2023
概括
在子中产前缺氧-缺血损伤会增加脊髓血清素 (5-HT),导致过度兴奋的运动神经元和类似CP的运动缺陷. 在这些运动神经元中减少的5-HT1A受体表达可能会损害中央疲劳机制.
科学领域:
- 神经科学是一个神经科学.
- 发育神经科学的发展神经科学.
- 神经生理学 神经生理学
背景情况:
- 大脑 (CP) 涉及到发育中的中枢神经系统的损伤,导致运动障碍,如肌肉硬.
- 在子中产前缺氧缺血性 (HI) 损伤模仿CP,导致肌肉硬和脊髓血清素 (5-HT) 的升高.
研究的目的:
- 调查脊柱运动神经元 (MN) 的改变的血清激素调制是否有助于产前HI损伤后的运动缺陷.
- 确定5-HT对HI受影响的子中MN兴奋性和功能的特定影响.
主要方法:
- 新生子脊髓切片 (出生后的0-5天) 上的ex vivo全细胞补丁电生理学.
- 应用5-HT受体激动剂 (α-甲基5-HT) 和再吸收抑制剂 (citalopram) 来评估MN反应.
- 在腰椎MNs中对5-HT1A受体进行免疫化.
主要成果:
- 与对照MN不同,HI子MN对5-HT表现出增强的刺激反应,包括增加的持续向内流和超极化的动作潜能值,与对照MN不同.
- 5-HT调节的曲/超极化激活的阴离子电流 (Ih) 和尖峰频率适应,特别是在HI电机受影响的MNs中.
- 与对照组相比,较少的HI子MNs表达了抑制性5-HT1A受体,这表明中央疲劳机制的潜在缺陷.
结论:
- 在产前HI损伤后脊柱5-HT升高直接增加了MN刺激能力,导致肌肉硬和性,这是CP的特征.
- 在HIMNs中,改变了5-HT受体表达,特别是减少了5-HT1A,可能导致过度兴奋和运动控制受损.
- 向血清能通路为患有脑膜炎的人提供了潜在的治疗策略.
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