在食道状细胞癌中,MUC1通过降低DNAJB6表达的调节来促进淋巴结转移
Guanqiang Ma1, Xiangyan Liu2, Mo Shi2
1Department of Thoracic Surgery, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.
Environmental toxicology
|August 16, 2023
概括
素1 (MUC1) 通过通过AKT-HSF-1通路降低DNAJB6的调节,促进食道状细胞癌 (ESCC) 的进展. 向MUC1抑制ESCC的生长和转移,提供了一个潜在的治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 异常的真1 (MUC1) 表达与食道状细胞癌 (ESCC) 的进展有关.
- 了解MUC1在ESCC恶性瘤中的作用对于开发向疗法至关重要.
研究的目的:
- 研究用Go-203准MUC1对ESCC恶性行为的影响.
- 阐明涉及DNAJB6和AKT/HSF-1通路的潜在分子机制.
主要方法:
- 免疫组织化学 (IHC) 来评估ESCC组织中的MUC1和DNAJB6表达.
- 在ESCC细胞系中进行基因和蛋白质表达分析的定量实时PCR (qRT-PCR) 和西部涂抹.
- 细胞计数工具-8 (CCK8),伤口愈合和Transwell测试以评估扩散,迁移和入侵.
- 西方涂抹以确定对AKT/HSF-1信号通路的影响.
主要成果:
- 在ESCC组织中观察到DNAJB6和MUC1表达之间的负相关性;高MUC1/低DNAJB6与淋巴结转移相关.
- MUC1过度表达促进了ESCC的扩散,入侵和迁移,同时降低了DNAJB6的调节,并激活了AKT通路.
- 用Go-203准MUC1抑制了ESCC的进展,调高了DNAJB6的调节,并抑制了AKT通路.
- MUC1通过AKT增强了HSF-1的酸化,并且抑制AKT-HSF-1增加了DNAJB6的表达.
结论:
- 通过通过AKT-HSF-1信号通路抑制DNAJB6表达,MUC1促进ESCC瘤发生和转移.
- 向MUC1代表了ESCC治疗的一个有前途的治疗策略.
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