准miR-146b-5p调节KDM6B表达加剧支气管肺发育不良症
YunFeng Long1, Yong Luo1, Liu Hu1
1Department of Neonatology, The First Affiliated Hospital of Shaoyang University, No. 39, Tongheng Street, Shuangqing District, Shaoyang City, 422000, Hunan Province, China.
Molecular biotechnology
|August 16, 2023
概括
微RNA-146b-5p (miR-146b-5p) 通过激活Wnt通路,在早产婴儿中加剧支气管肺功能障碍 (BPD). 抑制miR-146b-5p可能为BPD提供治疗策略.
科学领域:
- 新生儿医学 新生儿医学
- 分子生物学分子生物学
- 肺部研究 肺部研究
背景情况:
- 支气管肺功能障碍症 (BPD) 是一种慢性肺部疾病,会影响早产婴儿.
- 微RNA-146b-5p (miR-146b-5p) 在BPD病变发生过程中的作用尚未完全理解.
研究的目的:
- 在BPD模型中研究miR-146b-5p的表达和功能.
- 阐明miR-146b-5p影响BPD的分子机制.
主要方法:
- 已建立的BPD小鼠和MLE-12细胞模型在高氧化状态下.
- 使用HE,TUNEL,LDH,MMT和流细胞计评估肺损伤,炎症和亡.
- 通过光酶和RIP测试调查了miR-146b-5p针对KDM6B和Wnt通路激活的目标.
主要成果:
- 在BPD模型中,miR-146b-5p是上调的,而KDM6B是下调的.
- miR-146b-5p敲击改善了高氧化引起的肺损伤,炎症和亡.
- miR-146b-5p通过准KDM6B激活了Wnt通路,而KDM6B恢复逆转了这一效应.
结论:
- miR-146b-5p促进高氧诱导的肺上皮细胞炎症和BPD中的亡.
- 通过直接准KDM6B,miR-146b-5p激活了Wnt通路.
- 向miR-146b-5p可能是BPD的潜在治疗方法.
相关概念视频
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
02:23Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K