凝聚性RNA局部化和转录活动的SAM68调节粘附和subendothelial基底膜组件
Zeinab Rekad1, Michaël Ruff1, Agata Radwanska1
1Université Côte d'Azur, CNRS, INSERM, iBV, Nice, France.
eLife
|August 16, 2023
概括
RNA结合蛋白SAM68调节内皮细胞的粘附和运动,这对于形成新血管至关重要. 这一发现突出了SAM68的重要意义.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 血管生物学 血管生物学
背景情况:
- 内皮细胞与细胞外基质的相互作用对于血管的形成和维护至关重要.
- RNA结合蛋白 (RBPs) 在整合素粘附复合体中发现,但它们的作用在很大程度上是未知的.
- 与68kDa (SAM68) 线粒分裂相关的Src是一种RBP,其在内皮细胞中的功能需要研究.
研究的目的:
- 研究RNA结合蛋白SAM68在内皮细胞中的作用.
- 了解SAM68对细胞矩阵相互作用,运动性和血管生成的贡献.
主要方法:
- 在内皮细胞中探索SAM68的定位和功能.
- 分析SAM68对整合素信号传递,mRNA传递和细胞矩阵相互作用的影响.
- 评估SAM68在3D发芽血管生成模型中的作用.
主要成果:
- SAM68定位在细胞边缘,促进膜突起和焦点粘附成熟.
- SAM68调节整合素信号传递并提供β-actin mRNA,影响细胞矩阵相互作用和运动性.
- SAM68的枯竭会影响矩阵基因表达,并损害尖端和茎细胞中的生长血管生成.
结论:
- SAM68是一种新型RNA结合蛋白,参与调节内皮细胞粘附和运动.
- SAM68在血管恒温和血管生成的动态过程中发挥着重要作用.
- SAM68是血管网络形成的关键调节剂.
相关概念视频
Intracellular Signaling Affects Focal Adhesions
2.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
2.7K
Immunoglobulin-like Cell Adhesion Molecules
3.3K
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
3.3K
Adherens Junctions
4.9K
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
Adherens Junctions are Dynamic
4.9K
Leaky Scanning
5.2K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.2K
Cell Adhesion Molecules - Types and Functions
6.8K
Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily involved...
CAM Families
The Integrin family of proteins is primarily involved...
6.8K
Anchoring Junctions
3.8K
Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
3.8K


