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使用ITS2和COI序列对Anopheles culicifacies物种复合物的遗传多样性,系谱和系谱分析
R A S Rathnayake1, W M M Wedage2, L S Muthukumarana1
1Department of Zoology, Faculty of Applied Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka.
PloS one
|August 16, 2023
概括
阿诺菲莱斯 (Anopheles culicifacies) 蚊子物种复合体显示出显著的遗传多样性,其独特的分类和多样化可以追溯到2000万多年前. 最近斯里兰卡的疟疾控制工作可能影响了蚊子的遗传多样性.
科学领域:
- 遗传学和进化生物学
- 医学昆虫学 医学昆虫学
- 分子遗传学分子遗传学
背景情况:
- 阿诺菲莱斯 (Anopheles culicifacies) 是南亚的主要疟疾载体,存在于五个兄弟物种 (A-E) 的复合体中.
- 了解这一复合体内的遗传关系和多样性对于有效的疟疾控制策略至关重要.
研究的目的:
- 调查Anopheles culicifacies兄弟物种之间的遗传学和遗传地理关系.
- 评估亚洲种群中Anopheles culicifacies物种复杂体内的遗传多样性和差异化.
主要方法:
- 在公共数据库中的35个ITS2和77个COI序列上使用贝叶斯概率树进行了家族遗传学分析.
- 在BEAST中使用贝叶斯模型来估计分歧时间的植物地理分析.
主要成果:
- ITS2序列揭示了两个主要分类 (A,D和B,C,E).
- COI序列分别在遗传学和地理学上确定了四个和三个类.
- 多样化的估计范围从20.2524.12 Mya (ITS2) 和22.3726.22 Mya (COI),与物种A和D多样化最近.
- 斯里兰卡人群中的低分类型多样性表明疟疾控制的影响;远距离的人群之间观察到高遗传差异.
结论:
- 尽管采取了载体控制措施,但Anopheles culicifacies复合体表现出相当大的遗传多样性,表明了弹性.
- 兄弟物种之间存在着不同的家族遗传和家族地理模式,分离时间各不相同.
- 遗传数据提供了对人口结构的见解,以及对载体种群的控制努力的潜在影响.
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