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卡尔斯伯格公司为在家禽业中应用Carlsberg subtilisin的固定和对接研究

Anum Munir Rana¹, Bart Devreese², Stijn De Waele²

¹Department of Microbiology, Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

|August 16, 2023

概括

来自Bacillus licheniformis PB1的固定卡尔斯伯格微素提高了肉生长率15.03%. 这种蛋白酶在本托尼特上稳定,为改善动物生产提供了有前途的料补充剂.

科学领域:

酶学是一种酶学.
动物营养动物营养
生物技术是生物技术.

背景情况:

细菌菌PB1产生卡尔斯伯格亚素,一种具有作为料补充剂潜力的蛋白酶.
在bentonite等支上的酶固定可以提高稳定性和可用性.

研究的目的:

为了调查卡尔斯伯格公司在本托尼特上固定化的细素作为 brojlers的料补充剂.
为了评估这种酶复合体对肉生长率的影响.

主要方法:

蛋白酶提取,表征 (SDS-PAGE,MALDI-TOF-MS/MS) 和活动优化.
卡尔斯伯格亚提利辛的固定在顿石上,并评估其稳定性.
补充肉的料与subtilisin-bentonite复合体和生长性能评估.

主要成果:

卡尔斯伯格微素被描述为一种性蛋白酶,在50°C和pH值8.0时具有最佳活性.
在本托尼特上固定导致高活动保留 (86.39%) 和稳定性,在室温下两周后仅损失5%.
补充了亚提利辛-本托尼特复合物的肉显示体重增加显著增加15.03%.

结论:

在本托尼特上固定卡尔斯伯格亚提利辛是一种稳定有效的料补充剂.
这种酶复合物显著改善肉的生长性能.
该研究强调了酶技术在提高动物料效率方面的潜力.

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