菲拉胺A和B蛋白在调节菲洛病毒进入过程中的对比作用
Ariel Shepley-McTaggart1, Jingjing Liang1, Yang Ding1
1Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America.
PLoS pathogens
|August 16, 2023
概括
胺A和B蛋白调节埃博拉病毒 (EBOV) 和马尔堡病毒 (MARV) 进入宿主细胞. 胺A敲击抑制病毒的进入,而胺B敲击增强了它,揭示了新的治疗点.
科学领域:
- 病毒学 病毒学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 埃博拉病毒 (EBOV) 和马尔堡病毒 (MARV) 是导致高死亡率的严重出血发烧的细菌病毒.
- 了解filovirus与宿主之间的相互作用对于开发有效治疗方法至关重要.
- 菲洛病毒进入宿主细胞的主要介导方式是依赖于actin的巨细胞,涉及病毒糖蛋白 (eGP和mGP).
研究的目的:
- 为了确定调节EBOV和MARV输入的宿主细胞因素.
- 为了研究actin结合蛋白的作用,特别是filamine A (FLNa) 和filamine B (FLNb),在filovirus生命周期.
- 阐明FLNa和FLNb影响病毒进入的机制.
主要方法:
- 使用了淘汰细胞模型 (FLNaKD和FLNbKD) 来评估病毒进入.
- 伪型病毒 (psVSV-RFP-eGP) 和感染性重组病毒 (rVSV-eGP-mCherry) 的测试进入.
- 真正的EBOV和MARV在没有丝的细胞中进行了评估.
主要成果:
- 胺A敲除剂 (FLNaKD) 显著抑制了EBOV和MARV的进入.
- 菲拉B敲除剂 (FLNbKD) 令人惊的是增加了EBOV和MARV的进入.
- FLNa和FLNb对巨细胞细胞的不同调节似乎是它们对病毒进入的不同影响的基础.
结论:
- 菲拉明A和B被确定为EBOV和MARV输入的新型调节剂.
- FLNa和FLNb在介导filovirus进入细胞中的作用相反.
- 这些发现为开发针对EBOV和MARV感染的对策提供了潜在的新策略.
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