相关实验视频

Updated: Jul 19, 2025

Advances in Human Induced Pluripotent Stem Cell-Derived Chimeric Antigen Receptor-Expressing Natural Killer Cells

Advances in Human Induced Pluripotent Stem Cell-Derived Chimeric Antigen Receptor-Expressing Natural Killer Cells

Published on: February 14, 2025

NKG2D-CAR T细胞在老老鼠和非人类灵长类动物中消除衰老细胞

Dong Yang¹, Bin Sun¹, Shirong Li¹

¹Department of Targeting Therapy and Immunology and Laboratory of Animal Tumor Models, Cancer Center and State Key Laboratory of Biotherapy and National Clinical Research Center for Geriatrics and Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.

Science translational medicine

|August 16, 2023

概括

自然杀手组2成员的D配体 (NKG2DLs) 在衰老细胞中被上调调节. 针对NKG2DL的化学抗原受体 (CAR) T细胞有效地消除衰老细胞,使组织再生,治疗与年龄相关的疾病.

科学领域:

老年病学和免疫学
细胞生物学和衰老研究研究

背景情况:

细胞衰老,一种稳定的细胞循环停止状态,与衰老和与年龄相关的疾病有关.
消除衰老细胞已经显示出在再生组织和减轻与年龄相关的病理方面的潜力.

研究的目的:

为了研究自然杀手组2成员D联体 (NKG2DLs) 在细胞衰老中的作用.
开发和评估化抗原受体 (CAR) 向NKG2DL的T细胞作为老化疗法.

主要方法:

在各种诱导方法和体内模型 (老年小鼠,非人类灵长类动物) 中识别NKG2DLs在衰老细胞中的上调调节.
开发NKG2D-CAR T细胞,准人类NKG2DLs,对衰老细胞进行选择性消除.
在衰老和衰老模型中对CAR T细胞进行体外和体内测试.

主要成果:

在衰老细胞中,NKG2DLs的调节一直在上升,而不管是诱导衰老的刺激.
在体外,NKG2D-CAR T细胞可以选择性有效地消除衰老细胞.
在小鼠中用NKG2D-CAR T细胞治疗改善了衰老病态和改善了身体功能.
自主NKG2D CAR T细胞安全清除了老年非人类灵长类动物中自然存在的衰老细胞.

结论:

NKG2DLs是衰老细胞的可靠标记物.
NKG2D-CAR T细胞代表了一个有前途和选择性的老化疗法.
这种方法有可能用于治疗衰老和衰老驱动的疾病.

更多相关视频

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies

Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies

Published on: February 15, 2021

相关概念视频

Replicative Cell Senescence

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...

Cells of the Innate Immune Response

Cells of the Innate Immune Response

The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...