在光学显微镜图像中使用YOLOv5x自动检测Cryptosporidium:一项比较研究
Johan Sebastian Lopez Salguero1,2, Melissa Rodríguez Rendón1,2, Jessica Triviño Valencia2
1Departamento de Física y Matemáticas, Universidad Autónoma de Manizales, Antigua Estación del Ferrocarril, Manizales, CP 170001, Colombia.
Biochemistry and cell biology = Biochimie et biologie cellulaire
|August 16, 2023
概括
这项研究表明YOLOv5可以使用显微镜图像检测Cryptosporidium. YOLOv5s模型为寄生虫检测提供了精度和计算效率的最佳平衡.
科学领域:
- 微生物学 微生物学
- 计算机科学 计算机科学
- 寄生虫学的寄生虫学
背景情况:
- 杆菌是导致腹疾病的重要原因之一.
- 准确检测Cryptosporidium对于诊断和公共健康至关重要.
- 目前的检测方法可能是劳动密集型的,需要专门的专业知识.
研究的目的:
- 为了评估机器学习工具YOLOv5的有效性,用于检测Cryptosporidium.
- 用光学和相对比显微镜图像来比较YOLOv5的性能.
- 为了确定用于Cryptosporidium检测的最佳YOLOv5模型配置.
主要方法:
- 使用Python库进行图像预处理 (标签,大小调整,裁剪).
- 在光学 (520张图像) 和相对比 (1200张图像) 显微镜数据集上训练了YOLOv5 (s,m,l) 模型.
- 使用平均平均精度,混矩阵和F1分数评估模型性能.
主要成果:
- 最优的模型结合了光学成像训练和相对照成像再训练.
- 在光学成像中随机初始化的重量产生了最低的检测精度.
- YOLOv5m显示出最高的稳定性,但由于计算成本较低,建议使用YOLOv5s.
结论:
- YOLOv5是一个可行的工具,用于从显微镜图像中自动检测Cryptosporidium.
- YOLOv5s模型为寄生虫检测提供了一个高效的解决方案,平衡性能和计算资源.
- 进一步优化可以提高检测准确性和扩大临床适用性.
相关概念视频
Immunofluorescence Microscopy
10.7K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
10.7K
Imaging Biological Samples with Optical Microscopy
4.8K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.8K
Two-Dimensional Microscopy in Microbiology
81
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
81
Confocal Fluorescence Microscopy
13.4K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.4K
Phase Contrast and Differential Interference Contrast Microscopy
8.2K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
8.2K
Super-resolution Fluorescence Microscopy
7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K


