白海十头 (罗斯) O.Deg. 提取物通过调节TNF/Akt/NF-κB通路产生抗炎作用
Wen-Sha Meng1, Jia Sun2, Yuan Lu2
1State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou Medical University, Beijing Road 9, Guiyang 550004, PR China; School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550004, PR China.
概括
白白的十头提取物 (BDE) 通过向TNF-α来抑制TNF/Akt/NF-κB通路,显示出抗炎作用. 这项研究阐明了BDE治疗炎症的机制及其潜在的治疗应用.
科学领域:
- 药理学 药理学是指药理学的学科.
- 免疫学 免疫学 免疫学
- 自然产品 化学 化学
背景情况:
- Biancaea decapetala (Fabaceae) 传统上用于炎症状况,如感冒,发烧和类风湿性疼痛.
- 尚不完全了解Biancaea decapetala提取物 (BDE) 的精确抗炎机制.
研究的目的:
- 评估BDE的体外和体外抗炎活性.
- 研究BDE抗炎作用的潜在分子机制和潜在分子点.
主要方法:
- 在LPS刺激细胞和老鼠中评估氧化 (NO) 和细胞因子的释放.
- 利用转录组分析 (RNA-seq) 来识别BDE调节的途径.
- 使用RT-qPCR,西部涂抹,ELISA和免疫光检测来验证蛋白质表达.
- 应用表面等离子体共振 (SPR) 和药物亲和反应目标稳定性 (DARTS) 来确认目标结合和功能测试,以评估TNF-α/NF-κB通路抑制.
主要成果:
- BDE显著降低了TNF-α,IL-1β,IL-6和NO的水平,并改善了肺组织病理.
- RNA-seq数据表明,BDE调节了TNF/Akt/NF-κB通路.
- BDE降低了关键炎症媒介和参与TNF/Akt/NF-κB信号级联的蛋白质的下调,包括p-p65,p-Akt和p-IκBα.
- 实验证实BDE与TNF-α结合,抑制其活性和下游NF-κB信号传递.
结论:
- 通过向TNF-α和抑制TNF/Akt/NF-κB通路,BDE具有抗炎作用.
- 这些发现为BDE的抗炎性质提供了机械的理解.
- 这项研究为Biancaea decapetala的治疗开发提供了科学基础.
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