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通过负调节Adamts5,Eif2s3y减轻了小鼠丸LPS诱导的损伤,并维持了精子生成

Yunxiang Li¹, Wenping Wu¹, Wenjing Xu¹

¹College of Veterinary Medicine/Shaanxi Centre of Stem Cells Engineering & Technology, Northwest Agriculture & Forestry University, Yangling, Shaanxi, China.

|August 16, 2023

概括

细胞转化启动因子2,亚单元3,Y-linked (Eif2s3y) 保护小鼠丸免受炎症,并维持精子的生产. 它通过抑制Adamts5和TLR4/NFκB通路来实现这一目标,这对于精子生成至关重要.

科学领域:

生殖生物学生殖生物学
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

精子生成依赖于Eif2s3y基因 (真核体翻译启动因子2,亚单元3,Y链接).
Eif2s3y促进精子干细胞 (SSC) 的增殖,并可以促进SSC的分化成精子.
在丸炎症中Eif2s3y的作用及其保护机制仍然不完全理解.

研究的目的:

在小鼠中研究Eif2s3y过度表达对脂聚糖 (LPS) 诱导的丸炎症的保护作用.
阐明Eif2s3y在丸中发挥其保护功能的分子机制.

主要方法:

过度表达Eif2s3y在小鼠丸通过lentiviral注射.
使用腹腔内LPS注射诱导丸炎症.
基因和蛋白质表达的分析使用RNA测序,qRT-PCR和西方涂料.

主要成果:

Eif2s3y过度表达缓解了LPS诱导的丸损伤,并保留了精子生成.
经过Eif2s3y过度表达的试验在LPS暴露后表现出更有组织的精卵管.
Eif2s3y负调节了Adamts5 (一种含有血栓松丁-1动机的分解蛋白和金属蛋白酶),一种细胞外矩阵降解酶,并抑制了TLR4/NFκB信号通路.

结论:

Eif2s3y对LPS诱导的丸损伤起着至关重要的保护作用.
在炎症期间,Eif2s3y通过降低Adamts5和TLR4/NFκB通路的调节来维持精子生成.

关键词:

亚当斯5的人类如果2s3yy 在LPS中使用LPS.男性的生殖细胞是男性生殖细胞.精子发生是精子生成.测试 (Testis) 是一个测试.

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Spermatogenesis

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Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...