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基因型检测可以更好地检测人类简单疹病毒中的抗病毒耐药性

Heather L Glasgow¹, Haiying Zhu¹, Hong Xie¹

¹Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, 98195, United States.

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

|August 16, 2023

概括

对人类简单疹病毒 (HSV) 耐药性的基因型检测显示出有希望的结果,但需要改进. 结合基因型和表型测试,可以更准确和更快速地诊断免疫功能低下患者的HSV抗病毒耐药性.

科学领域:

病毒学病毒学
传染性疾病传染性疾病
分子诊断学分子诊断

背景情况:

人类简单疹病毒 (HSV) 的抗病毒耐药性构成了重大的临床挑战,特别是在免疫受损的个体中.
目前的HSV耐药性测试依赖于缓慢的,表型的,基于培养的方法,从而导致经验性的治疗决定.
对于许多病毒,分子诊断方法是先进的,但HSV耐药性测试落后.

研究的目的:

评估HSV基因型抗病毒耐药性测试的性能.
为了比较基因型测试与传统的表型斑块减少试验 (PRA).
评估基因型与表型HSV耐药性检测的准确性和速度.

主要方法:

采用了HSV菌株的全基因组测序.
使用了已知的阿西克洛维尔和福斯卡内特抗药突变的数据库.
基因型结果与内部和参考实验室的表型PRA对照菌株和临床隔离物进行了比较.

主要成果:

基因型和表型测试之间的一致性各不相同,观察到不确定的和不一致的结果.
不确定的结果通常是由于不确定的意义 (VUS) 的变异.
对表型敏感菌株的不一致结果揭示了潜在的耐药性突变,有时在耐药亚群中,重复测试改善了一致性.

结论:

基因型检测,当与表型方法相结合时,可以提高HSV抗病毒耐药性诊断的准确性和速度.
突变的基因型背景和病毒培养限制可能会影响表型耐药性测定结果.
作为HSV耐药性测试的黄金标准,单独的表型斑块减少试验具有局限性.

关键词:

阿西克洛维尔 (acyclovir) 是一种药物.抗病毒耐药性抗病毒耐药性基因型检测是指基因型检测.简单的疹病毒简单的病毒.减少斑块的测试.病毒序列测序病毒序列测序

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