干眼疾病和梅博米腺功能障碍的眼睛微生物组变化
Jerome Ozkan1, Marwan E Majzoub2, Minas Coroneo3
1School of Optometry and Vision Science, University of New South Wales, Sydney, Australia; Centre for Marine Science and Innovation and School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney, Australia.
Experimental eye research
|August 16, 2023
概括
干眼病 (DED) 和梅博米腺功能障碍 (MGD) 与眼睛表面细菌的变化有关. 这项研究在DED/MGD患者中发现了独特的微生物群落,女性的多样性减少.
科学领域:
- 眼科医生 眼科 眼科
- 微生物学 微生物学
- 基因组学就是基因组学.
背景情况:
- 干眼病 (DED) 和梅博米腺功能障碍 (MGD) 是与衰老相关的常见慢性眼病.
- 眼睛表面微生物组在维持平衡中起着重要作用,其干扰可能会导致疾病的发展.
研究的目的:
- 为了比较轻度至中度DED/MGD和健康对照个体的结膜和眼边缘微生物群.
- 研究眼功能障碍对DED/MGD患者眼部微生物组的影响.
主要方法:
- 从三个队列 (MGD,MGD带有眼功能障碍[MGD+LD]和对照组) 的结膜和眼边缘拭子的16S rRNA基因测序.
- 使用生物信息学管道进行序列处理,集群 (ASV) 和分类学分类 (BCLA与GTDB 2207对比) 的微生物群落分析.
主要成果:
- 在MGD,MGD+LD和对照组之间观察到总体微生物群体组成的显著差异.
- 与对照组相比,DED/MGD组在微生物群落中表现出更大的个体间变异性.
- 与男性相比,女性的细菌丰富度和多样性减少.
- 在MGD+LD组中,特定的细菌种群,包括Pseudomonas和Caballeronia,更丰富,而在MGD组中,Corynebacterium物种的数量更高.
结论:
- 与对照人群相比,DED/MGD患者的眼表面和眼边缘微生物群显著不同.
- 增加某些细菌的丰富性,包括脂性物种,与DED/MGD有关.
- 女性DED/MGD患者在眼睛表面表现出减少的细菌丰富性和多样性.
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