一种简单快速的替代方法,在冷保存后从精液中去除糖醇
Hsiu-Lien Herbie Lin1, Isabelle Grasseau2, Pascal Mermillod2
1CNRS, IFCE, INRAE, Université de Tours, PRC, 37380, Nouzilly, France; Division of Physiology, LRI, COA, 71246, Tainan, Taiwan.
Cryobiology
|August 16, 2023
概括
一种新的糖去除溶液 (GRS) 有效地从冷保存的精子中去除糖,改善了在没有专门设备的情况下人工授精后的生育率.
科学领域:
- 生殖生物学 生殖生物学
- 动物科学动物科学
- 冰冷保存技术 冰冷保存技术
背景情况:
- 标准的甘油度 (11%) 精冷保存可以导致生育问题,即使在2%,需要在人工授精 (AI) 之前去除甘油.
- 传统的连续稀释然后离心 (SDC) 去除糖是耗时的,需要专门的设备,包括冷藏室.
研究的目的:
- 开发一种简单,高效和具有成本效益的方法,从冷保存的精子中去除糖醇.
- 评估一种新的基于Percoll的糖醇去除溶液 (GRS) 与糖糖一起用于减轻冷解精子中的透应激.
主要方法:
- 开发和测试一种基于Percoll的糖去除溶液 (GRS),其成分各不相同 (GRS A,B,C,D).
- 在不同离心温度 (4°C和20°C) 下对体外精子参数进行GRS性能比较.
- 与传统的SDC方法相比,对最有前途的GRS配方 (GRS A和D) 的生育能力评估.
主要成果:
- 与GRS D相比,GRS A的生育率在4°C和20°C (p <0.05) 均显著更高.
- 通过GRS A获得的生育率与传统的SDC方法相比或高于.
- 这种新的GRS协议可以节省44%的时间,并且在灵活的温度 (4°C或20°C) 和简单的设备下有效运行.
结论:
- 基于Percoll的新型糖去除溶液 (GRS) 为精子冷藏银行提供了一个有价值的替代方案.
- 这种方法提高了生育结果,同时简化了冷保存的精子的解后处理.
- 该GRS协议代表了鸟类精子冷保存的重大进步,为AI程序提供了实际的好处.
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