Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.2K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.2K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.7K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.7K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.8K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.8K
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

2.0K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
2.0K
Forced Transdifferentiation01:28

Forced Transdifferentiation

1.9K
Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial...
1.9K
iPS Cell Differentiation01:22

iPS Cell Differentiation

2.7K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

AI-Accelerated Structure Elucidation of Boavistamides A-C, Cyclic Depsipeptides from a Marine Filamentous Cyanobacterium Collected in Cabo Verde.

bioRxiv : the preprint server for biology·2026
Same author

Mediating role of white blood cells in the relationship between the advanced lung cancer inflammation index and serum neurofilament light chain levels: A study based on NHANES 2013-2014 data.

Medicine·2026
Same author

AI-Accelerated Structure Elucidation of Boavistamides A<b>-</b>C, Cyclic Depsipeptides from a Marine Filamentous Cyanobacterium Collected in Cabo Verde.

Journal of natural products·2026
Same author

DNA methylation reprogramming in marsupial embryos is restricted to the extraembryonic lineage.

Nature communications·2026
Same author

Mesenchymal transitions reduce lamin A expression and nuclear stiffness to enhance confined migration in glioblastoma.

Scientific reports·2026
Same author

Gene body methylation suppresses intragenic transcription and permits epigenetic inheritance in a cnidarian.

Nature ecology & evolution·2026
Same journal

Family of magnetic field-boosted superconductors in rhombohedral graphene.

Nature·2026
Same journal

What's the human cost of US research turmoil? A new film finds out.

Nature·2026
Same journal

Daily briefing: Ovaries start a second job after menopause.

Nature·2026
Same journal

Audio long read: Is the peptide craze backed by science? The promise behind the hype.

Nature·2026
Same journal

Scientists fight back against far-right plans to restrict academic freedom in Germany.

Nature·2026
Same journal

How AI can crack open the 'hidden curriculum' for neurodivergent students.

Nature·2026
查看所有相关文章

相关实验视频

Updated: Jul 19, 2025

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
07:18

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Published on: May 12, 2017

6.5K

暂时的天真重编程可以在功能和表观遗传上纠正hiPS细胞

Sam Buckberry1,2,3,4, Xiaodong Liu5,6,7,8,9,10,11, Daniel Poppe1,2

  • 1Harry Perkins Institute of Medical Research, QEII Medical Centre and Centre for Medical Research, The University of Western Australia, Perth, Western Australia, Australia.

Nature
|August 16, 2023
PubMed
概括
此摘要是机器生成的。

暂时无效治疗 (TNT) 重编程纠正人类诱导多能干细胞 (hiPS细胞) 的表观遗传记忆和异常,使它们更像人类胚胎干细胞 (hES细胞). 这种新方法提高了生物医学应用的hiPS细胞分化.

更多相关视频

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Na&#239;ve-like State with Improved Multilineage Differentiation Potency
09:07

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency

Published on: June 10, 2018

10.0K
Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
10:47

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Published on: October 28, 2011

15.5K

相关实验视频

Last Updated: Jul 19, 2025

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
07:18

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Published on: May 12, 2017

6.5K
Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Na&#239;ve-like State with Improved Multilineage Differentiation Potency
09:07

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency

Published on: June 10, 2018

10.0K
Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
10:47

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Published on: October 28, 2011

15.5K

科学领域:

  • 表观遗传学
  • 干细胞生物学
  • 重编程技术

背景情况:

  • 人类诱导多能干细胞 (hiPS细胞) 经历显著的表观基因组变化,但与人类胚胎干细胞 (hES细胞) 保持差异.
  • 这些表观遗传差异,包括记忆和异常,影响hiPS细胞功能,其潜在机制在很大程度上未知.

研究的目的:

  • 在hiPS细胞重编程期间表观遗传差异的出现和持续的特征.
  • 开发一种新的重编程策略,模拟胚胎表观遗传重置并纠正hiPS细胞表观遗传缺陷.

主要方法:

  • 在原始和纯粹的重编程过程中对全基因组DNA进行甲基化分析.
  • 开发和应用一个暂时无效处理 (TNT) 重编程策略.
  • 同源系统分析以评估表观遗传纠正和功能结果.

主要成果:

  • 重编程诱导的表观遗传异常发生在重编程的中期; DNA 脱甲基化在初始重编程中开始.
  • 通过TNT重编程,细胞的来源依赖性抑制色素 (H3K9me3,拉米-B1,CpH甲基化) 被重新配置为类似hES细胞的状态.
  • 与传统的hiPS细胞相比,TNT重编程的hiPS细胞表现出更正的可转移元素表达,改善的基因表达和增强的分化效率.

结论:

  • TNT重编程有效地纠正表观遗传记忆和异常,产生与hES细胞分子和功能相似的hiPS细胞.
  • 这种策略不会破坏基因组印记,并改善各种细胞类型的分化.
  • TNT重编程为生物医学和治疗应用提供了一个潜在的新标准,也是研究表观遗传记忆的工具.