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在Cryptocaryon irritans Tomont中,Honokiol诱导了类似亡的死亡
Zi-Chen Zhao1,2, Man-Yi Jiang1, Ji-Hui Huang1,3
1Hainan Provincial Key Laboratory for Tropical Hydrobiology and Biotechnology, College of Marine Science, Hainan University, Haikou, 570228, People's Republic of China.
Parasites & vectors
|August 16, 2023
概括
霍诺基奥尔通过破坏平衡和激活酶通路来诱导Cryptocaryon irritans Tomonts中的类似亡的死亡. 这为海鱼中C. irritans感染提供了潜在的新疗法.
科学领域:
- 海洋生物学 海洋生物学
- 寄生虫学的寄生虫学
- 水产养殖是水产养殖的一种方式.
背景情况:
- 克里普托卡里昂刺激虫 (Cryptocaryon irritans) 是一种影响海洋鱼类水产养殖的重要寄生虫.
- 之前的研究表明,香醇会诱导C. irritans致死,但机制尚不清楚.
研究的目的:
- 为了阐明何诺基奥尔诱导C. irritans托蒙特斯的亡性死亡的机制.
- 调查Honokiol对细胞细胞灭绝相关的细胞过程的影响.
主要方法:
- 使用显微镜和AnnexinV-FITC/PI染色的亡形态和比率评估.
- 测量细胞内 ([Ca2+]i),线粒体膜潜力 (ΔΨm),活性氧物种 (ROS),DNA碎片化和酶活性.
- 实时PCR分析了与亡相关的基因的表达.
主要成果:
- 霍诺基奥尔在1μg/ml的剂量有效诱导了类似亡的死亡,其特征是增加[Ca2+]i,ROS,caspase-3/9活性和DNA碎片化,死亡缩减少.
- 关键的细胞事件,包括升高的[Ca2+]i,ROS和酶活性,发生在治疗Honokiol的1-2小时内.
- 观察到14个基因的上调,特别是那些参与ER压力诱导的亡的基因.
结论:
- 霍诺基奥尔诱导了C. irritans托蒙特的类似亡的死亡.
- 这种机制涉及到细胞内平衡在细胞内网膜中被破坏,触发了酶级联或线粒体通路.
- 霍诺基奥尔为C. irritans感染提供了一个潜在的新疗法策略.
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