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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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通过AcrIIC4进行CRISPR-Cas9的抑制机制

Xuzichao Li1, Fumeng Liao1, Jiaqi Gao1

  • 1State Key Laboratory of Experimental Hematology, Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Haihe Laboratory of Cell Ecosystem, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.

Nucleic acids research
|August 17, 2023
PubMed
概括

反CRISPR蛋白AcrIIC4通过锁定其REC2域来抑制Neisseria meningitidis Cas9 (NmeCas9),防止DNA结合和裂变. 这一发现为CRISPR-Cas调节和基因编辑工具开发提供了洞察力.

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