m6A-Atlas v2.0:更新了在多个物种中解开N6-甲基氨酸 (m6A) 表谱组的资源
Zhanmin Liang1,2, Haokai Ye2,3, Jiongming Ma1,2,3
1Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian 350004, China.
Nucleic acids research
|August 17, 2023
概括
m6A-Atlas v2.0增强了对N6-甲基氨酸 (m6A) RNA修饰的研究. 这个更新的数据库提供了数以百万计的m6A位点和峰值,有助于研究这一关键的表皮转录体标记.
科学领域:
- 史诗转录组学 史诗转录组学
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- N6-甲基氨酸 (m6A) 是一种在真核生物中普遍存在的内部mRNA修饰.
- m6A在各种分子功能和生物过程中发挥着关键作用.
研究的目的:
- 为了介绍m6A-Atlas v2.0,一个更新的m6A研究的全面资源.
- 促进研究m6A修饰及其功能影响.
主要方法:
- 来自13种高分辨率技术和单细胞配置文件的综合数据.
- 应用了exomePeaks2,MACS2和TRESS来分析来自2712个MeRIP-seq实验的1600万m6A缩峰值.
- 进行了特定条件的定量m6A分析,用于差异甲基化分析.
主要成果:
- m6A-Atlas v2.0包括797,091个可靠的m6A站点和42个物种的超过1600万个m6A缩峰.
- 为172个条件提供了MeRIP-seq样本的质量控制指标和差异甲基化数据.
- 带有基因组特征的注释的m6A表谱学,RNA结合蛋白相互作用,miRNA,拼接和SNP.
结论:
- m6A-Atlas v2.0 作为一个有价值的,用户友好的资源来探索m6A的修改.
- 集成的数据和注释提供了关于m6A在表皮转录学中的功能作用的见解.
- 通过http://rnamd.org/m6a.org方便查询和下载m6A网站和功能注释.
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