基因重复促进了普通黄的C4-CAM兼容性
Xiaoliang Wang1,2,3,4, Xuxu Ma1,3,4,5, Ge Yan1,3,4,5
1Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Plant physiology
|August 17, 2023
概括
常见的鱼表现出C4和crassulacean酸代谢 (CAM) 光合作用. 基因组分析显示,两个全基因组重复 (WGD) 塑造了其独特的光合作用途径,为作物工程提供了洞察力.
科学领域:
- 植物生物学 植物生物学
- 基因组学就是基因组学.
- 光合作用研究研究光合作用.
背景情况:
- 常见的 (Portulaca oleracea) 独特地结合了C4和酸代谢 (CAM) 光合作用.
- 这种可塑性使其成为研究C4-CAM集成的有价值模型.
研究的目的:
- 为了生成一个高质量的基因组组合的普通.
- 为了研究这种物种中C4和CAM路径的进化历史.
- 为了确定C4-CAM可塑性背后的遗传机制.
主要方法:
- 染色体层次的基因组测序和组装.
- 遗传学分析和全基因组复制 (WGD) 测年.
- 基因复制数变异分析.
- 对基因促进体中cis调节元件的分析.
主要成果:
- 一个高质量的NAD-酶 (ME) 亚型常见 purslane的基因组被生产出来.
- 有两个WGD事件的证据:一个古老的P-β (66.30万年前) 和一个血统特定的Po-α (7.74万年前).
- Po-α WGD生成了C4光合作用C4光合作用C4光合作用的酸核糖酶基因拷贝.
- P-β WGD产生了功能差异化的β碳酸无水酶 (C4-和CAM-特定) 的祖先基因.
- 特定于CAM的基因招募了昼夜和乙烯反应因子 cis 元素.
结论:
- 这项研究阐明了普通鸟中C4和CAM通路的进化起源.
- WGD事件在扩大光合作用基因家族方面发挥了关键作用.
- 识别的监管要素提供了在作物中设计C4或CAM代谢的目标.
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