优化不同的生长参数,以最大限度地生产生物活性原始代谢物,由Aspergillus fumigatus
Sidra Farooq1, Iqra Fiaz2, Hamza Ahmad3
1Department of Health and Biological Sciences, Abasyn University, Peshawar, Pakistan.
Pakistan journal of pharmaceutical sciences
|August 17, 2023
概括
来自Aspergillus fumigatus的二次代谢产物具有强大的抗菌,抗真菌和杀虫性能. 这些化合物与抗生素具有有前途的协同作用,表明它们在各种应用中的潜力.
科学领域:
- 微生物学 微生物学
- 自然产品化学 自然产品化学
- 生物技术是生物技术.
背景情况:
- 阿斯伯吉洛斯烟虫产生各种具有潜在生物活性的二次代谢物.
- 研究这些代谢物对于发现新型抗微生物和杀虫剂至关重要.
研究的目的:
- 优化Aspergillus fumigatus的生长,以最大限度地生产生物质和二次代谢物.
- 评估A. fumigatus二次代谢产物的抗菌,抗真菌和杀虫作用.
- 评估这些代谢物与常规抗生素的协同作用.
主要方法:
- 为A. fumigatus.优化真菌生长条件 (介质,温度,pH,)
- 使用乙烯酸乙烯提取的二次代谢物.
- 通过抑制区和最小杀菌度 (MBC) 和最小抑制度 (MIC50) 评估的抗菌活性.
- 评估了对各种生物体的抗真菌,植物毒性和杀虫活性.
- 使用红外光谱分析了代谢物成分,并测试了与抗生素的协同作用.
主要成果:
- 在优化的条件下,在28°C,pH 4的土豆乳糖中产生了最大的生物质和代谢物,并进行了动.
- 代谢物对沙门氏菌 (Salmonella flexneri) 和型杆菌 (S. typhi) 具有显著的抗菌活性,对氏菌 (Penicillium chrysogenum) 和氏菌 (Aspergillus oryzae) 具有抗真菌活性.
- 这种提取物对Bactrocera cucurbitae,Sitotroga cerealella和Callosobruchus maculatus表现出强大的杀虫活性,并且具有显著的植物毒性.
- 红外光谱显示了,和的存在;代谢产物与抗生素有很好的协同作用.
结论:
- Aspergillus fumigatus的二次代谢物具有广泛的生物活性,包括抗微生物,杀虫剂和植物毒性作用.
- 这些代谢物是开发新型天然农药和治疗剂的潜在来源.
- 观察到的与抗生素的协同作用需要进一步研究,以加强治疗策略.
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