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采样,固定,软化和切割整个蜜蜂的实用方法,用于组织学评估
Sarah E Cook1,2, Bernardo D Niño3, Laura Rivera3
1Departments of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California-Davis, Davis, CA, USA.
概括
标准的甲固定和间接干冰暴露对于准备高质量的蜜蜂组织学样本是有效的. 这些方法可以确保对Apis mellifera的准确诊断,这对于兽医病理学和研究至关重要.
科学领域:
- 兽医病理学 兽医病理学
- 养蜂科学 养蜂科学 养蜂科学
- 组织学技术 组织学技术
背景情况:
- 西方蜜蜂 (Apis mellifera) 对农业和蜂巢产品至关重要.
- 兽医病理学培训往往缺乏全面覆盖蜜蜂解剖学和生物学.
- 标准化组织样本处理对于精确的蜜蜂诊断和研究至关重要.
研究的目的:
- 开发和评估处理蜜蜂样本用于组织学的现场协议.
- 为了比较各种固定,收集和软化方法的组织质量结果.
主要方法:
- 测试了七个协议,包括常规的甲固定,Nair或乙醇软化,多种固定时间,干冰暴露和长期冷藏.
- 从经过加工的Apis mellifera工蜂中评估组织质量的组织质量.
主要成果:
- 通过常规的甲固定,两天的奈尔暴露,间接的干冰暴露和早期组织修剪,实现了最高质量的组织学截面.
- 最差的结果来自5天的Nair暴露,3天的乙醇预固定和长时间的-80°C储存.
- 常规的甲固定是足够的;干冰固定有助于在不影响质量的情况下收集样品.
结论:
- 常规的甲固定与间接干冰暴露相结合,为蜜蜂组织学提供了最佳的结果.
- 这些经过验证的方法支持准确的诊断和研究结果在兽医病理学Apis mellifera.
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