LncRNA LINC00847通过调节 MiR-133a-3p/TGFBR1轴加速黑色素瘤的进展
Lei Jiang1, Xiufang Shi1, Yanxin Liu2
1Department of Aesthetic, Plastic and Burn Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, 264000, China.
Combinatorial chemistry & high throughput screening
|August 17, 2023
概括
长非编码RNA LINC00847 在皮肤黑色素瘤中高度表达,促进瘤的进展. 抑制LINC00847通过miR-133a-3p/TGFBR1通路抑制了黑色素瘤细胞的增殖和转移.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 长非编码RNAs (lncRNAs) 越来越多地被认为是它们在瘤发生中的调节作用.
- 最近发现的一种lncRNA,LINC00847,已与各种癌症有关,但其在皮肤黑色素瘤中的功能仍然基本不明.
研究的目的:
- 研究LINC00847在皮肤黑色素瘤的表达模式和功能作用.
- 阐明LINC00847参与黑色素瘤进展的分子机制.
主要方法:
- 使用TCGA数据库和临床样本在皮肤黑色素瘤组织和细胞系中的量化LINC00847表达.
- 利用RT-qPCR和西式斑点测试来评估基因和microRNA表达.
- 进行了体外测试 (CCK8,殖民地形成,伤口划伤,Transwell) 以评估细胞增殖,入侵和迁移.
- 采用生物信息学预测,双露西法酶记者测定和RNA免疫沉来确认分子相互作用.
主要成果:
- 与正常对照组相比,LINC00847表达在皮肤黑色素瘤组织和细胞系中显著上调.
- 敲除LINC00847明显抑制了皮肤黑色素瘤细胞的增殖,转移和伤口愈合.
- 确定了一个调节轴,其中LINC00847负调节miR-133a-3p,这反过来又针对TGFBR1.1.
结论:
- 在皮肤黑色素瘤中,LINC00847是致癌的,其过度表达促进了恶性行为.
- 该LINC00847/miR-133a-3p/TGFBR1通路在皮肤黑色素瘤的发病过程中起着至关重要的作用.
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