针对线粒体的分离剂通过增强ICAM1基因促进体甲基化来降低内皮细胞中的炎症反应
Liudmila A Zinovkina1, Ciara I Makievskaya1,2, Ivan I Galkin3,4
1Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 119991 Moscow, Russia.
Current molecular pharmacology
|August 17, 2023
概括
轻度线粒体解剂增加ICAM1基因促进物甲基化,减少炎症. 内皮细胞的表观遗传变化为心血管疾病提供了潜在的治疗策略.
科学领域:
- 细胞生物学 细胞生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 线粒体生物学 线粒体生物学
背景情况:
- 内皮炎症驱动心血管疾病.
- 线粒体调节内皮细胞的功能.
- 轻微的线粒体解显示出抗炎作用,但机制尚不清楚.
研究的目的:
- 调查线粒体离合器对内皮ICAM1基因促进物甲基化的影响.
- 探索线粒体解器抗炎活性背后的表观遗传机制.
主要方法:
- 研究的线粒体向解器:SkQ1,C12TPP和DNP.
- 通过RT-qPCR测量细胞间粘附分子1 (ICAM1) 的mRNA表达.
- 使用二硫酸盐测序评估ICAM1促进剂CpG位点甲基化.
主要成果:
- DNP和C12TPP降低了ICAM1的表达.
- 这种下调与增加的ICAM1促进剂CpG甲基化相关.
- 没有观察到细胞内或线粒体内ATP水平的显著变化.
结论:
- 线粒体离合剂增强ICAM1促进子甲基化,降低ICAM1mRNA水平.
- 对ICAM1促进体的表观遗传修饰可能会调解线粒体脱的抗炎作用.
- 线粒体解器通过DNA甲基化变化显示出对内皮炎症的治疗潜力.
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