质量/数量mNGS与通常的mNGS在疑似肺部感染中检测病原体的比较
Zhan Zhao1, Xuefen Chen1,2, Yubao Wang1
1Respiratory Department, Tianjin Medical University General Hospital, Tianjin, China.
Frontiers in cellular and infection microbiology
|August 17, 2023
概括
质量/数量元基因组下一代测序 (QmNGS) 显示出类似的灵敏度,但比通常的mNGS (UmNGS) 更高的特异性,用于诊断肺部感染. QmNGS是临床使用的一个可行的选择.
科学领域:
- 医学诊断 医学诊断 医学诊断
- 微生物学 微生物学
- 基因组学就是基因组学.
背景情况:
- 下一代元基因组测序 (mNGS) 越来越多地用于诊断肺病原体.
- 质量/数量mNGS (QmNGS) 是一种改进的方法,但与通常的mNGS (UmNGS) 的直接比较很少.
研究的目的:
- 在怀疑肺部感染的患者中,前性地比较QmNGS和UmNGS的诊断性能.
主要方法:
- 来自36名怀疑肺部感染的患者的支气管洗液使用UmNGS和QmNGS进行了分析.
- 评估了诊断性能指标,包括灵敏度,特异性和概率比率.
主要成果:
- 与UmNGS相比,QmNGS表现出类似的灵敏度和更高的特异性 (尽管在统计上并不显著).
- 对QmNGS的正概率为3.956和UmNGS的1.394;负概率分别为0.342和0.527.
- 病原体共检测一致性为77.2%,QmNGS显示的测序深度和覆盖范围低于UmNGS.
结论:
- QmNGS是诊断肺部感染的有希望的工具,比UmNGS具有可比的灵敏度和提高的特异性.
- 进一步的研究可能会优化QmNGS,用于在非肺部感染中全面检测病原体,包括细菌和真菌.
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