PORCN通过Wnt/β-catenin依赖的方式促进肝细胞致癌
Shuzhen Wu1, Zhaoxiu Liu1, Jing Chen1
1Department of Gastroenterology, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, China.
Translational cancer research
|August 17, 2023
概括
猪类O-转移酶 (PORCN) 通过激活Wnt-β-catenin通路促进肝细胞癌 (HCC) 细胞生长和扩散. 准PORCN可能为HCC患者提供新的治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 猪O-酸转移酶 (PORCN) 对于Wnt配体的棕化是必不可少的.
- 目前尚不清楚PORCN在肝细胞癌 (HCC) 发展中的作用.
研究的目的:
- 研究PORCN在HCC中的表达和预后意义.
- 阐明PORCN在HCC进展中的功能作用和分子机制.
主要方法:
- 定量聚合酶连锁反应 (qPCR),西部污染和癌症基因组图谱 (TCGA) 数据库分析.
- 在体外 (细胞计数套件-8,伤口愈合,Transwell测定) 和体内 (异种移植小鼠模型) 实验.
- 基因组丰富分析 (GSEA),免疫光染色和亚细胞蛋白质分离.
主要成果:
- 与正常组织相比,PORCN表达 (蛋白质和mRNA) 在HCC组织中升高.
- 在HCC患者中,较高的PORCN表达与总生存率 (OS) 降低相关.
- 过度表达PORCN增强了HCC细胞的增殖和迁移在体外和体内,涉及Wnt-β-catenin通路和上皮-介质细胞过渡 (EMT).
结论:
- PORCN通过Wnt-β-catenin信号通路促进HCC细胞的增殖和迁移.
- PORCN通过促进β-catenin的核转移来诱导EMT.
- PORCN代表了肝细胞癌的潜在治疗标.
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