先进的液体染色学与双重质谱法用于量化草甘,酸和氨基甲基酸使用前列衍生方法
Pedro J Martin1, Ke He2, Lee Blaney2
1Department of Civil & Environmental Engineering, Samueli School of Engineering, University of California, Irvine, Irvine, California 92697, United States.
概括
这项研究提出了一种新的方法,用于检测水中的草甘基除草剂,使用9-甲基甲酸 (FMOC-Cl) 衍生和LC-MS/MS分析. 优化的方法确保了草甘,葡萄酸盐和AMPA的准确量化.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
背景情况:
- 基于草甘的除草剂很难在水中检测,因为分析的局限性.
- 传统方法难以检测这些除草剂,因为它们缺乏染色体,并且是zwitterionic.
研究的目的:
- 开发一种简单有效的方法来量化水中的草甘,酸和氨基甲基酸 (AMPA).
- 优化使用9-甲基酸盐 (FMOC-Cl) 进行前列衍生,用于用双重质谱 (LC-MS/MS) 进行液体染色学.
主要方法:
- 使用2^4因子设计来优化关键导数变量.
- 使用的9-甲基甲酸 (FMOC-Cl) 用于列前衍生.
- 分析样品使用液态色谱与双重质谱法 (LC-MS/MS).
主要成果:
- 完整的衍生取决于性条件,由酸盐缓冲器为氨解提供.
- 1%的乙烯基二胺三酸 (EDTA) 对于最小化金属化和确保可重复结果至关重要,而更高的度则导致离子抑制.
- 在4小时后,通过2.5mM的FMOC-Cl实现了最佳衍生,避免了干扰检测的氧化物形成.
结论:
- 开发的方法提供了一种可靠的方法来量化水中的草甘,葡萄酸盐和AMPA.
- 缓冲组合,EDTA度,FMOC-Cl度和反应时间的优化对于准确检测除草剂至关重要.
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