在10种物种的TCGA数据集中,可转移元素衍生的微RNA表达模式
Chan-Mi Lee1, Sang Woo Jin2, Byunghyun Jang3
1Department of Otorhinolaryngology-Head and Neck Surgery, Korea University Guro Hospital, Seoul, South Korea.
Evolutionary bioinformatics online
|August 17, 2023
概括
可移植元素衍生的微RNA (miRNA) 显示与癌症相关的进化模式. 像miR-130a这样的保存miRNA和像miR-625这样的特定物种miRNA为癌症诊断和预后提供了洞察力.
科学领域:
- 进化生物学是进化的生物学.
- 基因组学就是基因组学.
- 癌症研究 癌症研究
背景情况:
- 微RNA (miRNA) 是疾病发病过程中的关键的非编码RNA调节剂.
- 了解与疾病相关的miRNAs的进化背景,特别是那些来自可移植元素 (TE) 的miRNAs,可以增强诊断和预后能力.
- 来自TE的miRNAs代表了探索疾病机制的潜在有价值领域.
研究的目的:
- 研究可移植元素衍生微RNA (TE-miRNAs) 与各种癌症相关的进化模式.
- 为了识别与癌症状态,阶段和生存相关的差异表达miRNAs (DEMs).
- 探索跨物种基因-miRNA相互作用的进化保存和特定物种的TE-miRNAs.
主要方法:
- 从癌症基因组图谱 (TCGA) 中对33种癌症中的34个miRNA进行比较分析.
- 基于癌症状态,阶段和存活率的统计比较 (t-测试) 来识别差异表达的miRNAs (DEMs).
- 使用TargetScan数据库和基因本体学 (GO) 丰富的基因-miRNA相互作用的跨物种 (10种) 分析.
主要成果:
- 在33种癌症中的18种中,至少有一种miRNA的显著差异表达 (P < .05, 倍数变化 > .05).
- 总共有128个涉及9个miRNA的DEM被确定,在所有10个物种中发现了保存的miR-130a,并确定了人类特异的miR-151a.
- 进化保存的miR-130a与脏和甲状腺癌有关;人类和 rhesus子特定的miR-625与各种癌症有关.
结论:
- 来自TE的miRNAs表现出与癌症发育和进展相关的进化保护模式.
- 特定的miRNAs,如保存的miR-130a和特定物种的miR-625,显示出某些癌症的生物标志物的潜力.
- 对TE-miRNAs的进化分析为癌症生物学提供了宝贵的见解,并可能有助于开发新的诊断和预后策略.
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