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研究丸细胞中与SARS-Cov-2相关的细胞因子和基于scRNA-seq数据的细胞之间的相互作用网络

Fan Yu¹, Haihong He², Tingting Huang¹

¹Center of Clinical Laboratory Medicine, Shenzhen Hospital, Southern Medical University, Shenzhen, Guangdong, 518000, China.

Open life sciences

|August 17, 2023

概括

COVID-19细胞因子风暴可能通过破坏丸细胞和改变免疫微环境来影响男性生育能力. 像IL8和CCL3这样的关键细胞因子在丸细胞发育和免疫反应中至关重要.

科学领域:

免疫学免疫学免疫学
生殖生物学生殖生物学
病毒学病毒学

背景情况:

COVID-19细胞因子风暴 (CS) 涉及炎症性细胞因子的升高.
通过丸损伤和免疫失调,CS可能会对男性的生育能力产生负面影响.
关于CS期间丸细胞因子表达和细胞通信的数据有限.

研究的目的:

为了研究COVID-19细胞因子风暴对丸细胞和男性生育能力的影响.
确定参与丸免疫过程和细胞通信的关键细胞因子.
分析丸微环境中的细胞因子相互作用网络.

主要方法:

丸细胞的综合单细胞测序数据.
用标记基因和人类细胞景观数据库对20个细胞群进行了注释.
使用CellChat构建了细胞伪时间轨迹,并分析了细胞因子相互作用网络.

主要成果:

在免疫过程中确定了12种丸细胞类型和4种细胞因子 (IL8,CCL2,CCL3,TNF).
IL8和CCL3在丸巨细胞和内皮细胞发育中起作用,影响免疫微环境.
在细胞相互作用网络中确定了八种关键细胞因子 (IL1,IL2,IL4,IL6,CCL,CSF3,TNF,IFN-II),IL2可能会影响所有丸细胞.

结论:

COVID-19的细胞因子风暴会破坏丸细胞的功能和通信.
特定的细胞因子与免疫反应和丸内的细胞发育有关.
研究结果提供了对COVID-19期间影响男性生育能力的机制的见解.

关键词:

在 COVID-19 疫情中,这就是SARS-CoV-2病毒.细胞因子细胞因子一个单细胞RNA-Seqq.丸细胞是丸细胞的一个组成部分.

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