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在口腔状细胞癌的甲固定嵌组织中对lncRNA的定量分析

Kiran Kumar¹, Kaveri Hallikeri¹, Ajaykumar Oli²

¹Department of Oral and Maxillofacial Pathology and Oral Microbiology, SDM College of Dental Sciences and Hospital (a constituent unit of Shri Dharmasthala Manjunatheshwara University), Dharwad, Karnataka State, 580009, India.

|August 17, 2023

概括

这项研究表明,使用实时定量PCR分析,可以使用甲固定型嵌 (FFPE) 组织对口腔状细胞癌中的调节性长非编码RNA (lncRNAs) 进行准确的定量分析.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

口腔状细胞癌 (OSCC) 是一种流行癌症.
调节性长非编码RNAs (lncRNAs) 在癌症发展中起着至关重要的作用.
分析lncRNA表达对于理解OSCC病原体至关重要.

研究的目的:

评估OSCC中调控性lncRNAs的表达特征.
为了比较新鲜冷和FFPE组织之间的RNA质量和表达分析.
评估使用FFPE组织在OSCC中用于lncRNA定量化的可行性.

主要方法:

使用来自OSCC和相邻正常粘膜的新鲜冷和FFPE组织.
分离了RNA,并使用实时定量PCR分析了lncRNAs的表达特征.
数据被规范化并与组织类型以及癌症基因组图谱 (TCGA) 数据库进行比较.

主要成果:

与新鲜冷组织相比,FFPE组织中的RNA数量和质量较低.
在配对组织之间没有观察到正常化lncRNA表达的显著差异.
不同的lncRNA表达模式与TCGA头部和部状细胞癌数据库一致.

结论:

使用OSCC.OSCC的FFPE样本,可以对lncRNAs进行准确的定量分析.
标准化的实时定量PCR测定用短片对FFPE样本有效.
在OSCC中,FFPE组织为回顾性lncRNA表达研究提供了一个可行的选择.

关键词:

用甲固定的氨酸嵌入的组织.新鲜的冷组织.在 lncRNAs 中.口腔状细胞癌的癌症.实时定量PCR实时定量PCR

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相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...