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密度梯度中性粒细胞隔离和基于流动细胞计的人类外周血液的表征的协议

Yi Kuang¹, Upasana Parthasarathy¹, Roberta Martinelli¹

¹Discovery Immunology, Merck & Co., Inc., Cambridge, MA 02141, USA.

|August 17, 2023

概括

从血液中分离关键免疫细胞中性粒细胞是一项挑战. 本协议详细介绍了一种密度梯度方法,用于获得纯中性粒细胞,用于对宿主免疫反应的研究.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

中性粒细胞是对病原体的宿主防御中的关键的第一响应者.
研究新鲜的中性粒细胞由于它们的敏感性和快速激活而带来了重大挑战.

研究的目的:

提出一种可靠的协议,用于从周围血液中分离中性粒细胞.
为了能够对中性粒细胞进行详细的形态和免疫表型分析.

主要方法:

使用密度梯度与周围血液分离来隔离中性粒细胞.
通过细胞素制剂进行形态评估.
使用流细胞测量分析进行免疫类型鉴定.

主要成果:

成功地从健康的捐赠者中分离出高纯度的中性粒细胞.
证明协议适用于健康和患病的个人.

结论:

这种密度梯度协议为中性粒细胞隔离提供了强大的方法.
隔离的中性粒细胞适合下游分析,有助于研究免疫反应.

关键词:

细胞生物学细胞生物学细胞隔离细胞隔离流量细胞计/质量细胞计.免疫学免疫学免疫学

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