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Updated: Jul 19, 2025

Author Spotlight: Integrating BRET-Based Assays and Rare Mutation Analysis to Decipher RAF Kinase Regulation in Live Cells
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使用基于近距离的NanoBRET测定方法测量活细胞中BRAF自身抑制的协议

Russell Spencer-Smith1, Deborah K Morrison1

  • 1Laboratory of Cell and Developmental Signaling, Center for Cancer Research, National Cancer Institute-Frederick, Frederick, MD 21702, USA.

STAR protocols
|August 17, 2023
PubMed
概括

研究人员开发了一种新的NanoBRET试验,可以实时在活细胞中测量BRAF自身抑制. 这种方法对于了解癌症和RASopathy综合征中的BRAF激活至关重要.

关键词:
癌症 癌症 癌症 癌症细胞生物学 细胞生物学基于细胞的测试蛋白质生物化学 蛋白质生物化学信号传输 信号传输

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