SMOC-1 与BMP和glypican相互作用,以调节C. elegans中的BMP信号传递
Melisa S DeGroot1, Byron Williams1, Timothy Y Chang1
1Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, United States of America.
PLoS biology
|August 17, 2023
概括
分泌的模块化结合蛋白 (SMOCs) 调节了C. elegans中的骨形态蛋白 (BMP) 信号传递. CeSMOC-1与LON-2/glypican和DBL-1/BMP结合,作为BMP通路的负和正调节者.
科学领域:
- 生物化学 生化学
- 分子生物学分子生物学
- 发展生物学 发展生物学
背景情况:
- 分泌的模块化结合蛋白 (SMOCs) 是保存的母细胞蛋白.
- SMOCs含有细胞外结合 (EC) 和甲状腺球蛋白-1型 (TY) 域.
- SMOCs影响骨形态遗传蛋白 (BMP) 信号通路.
研究的目的:
- 剖析C. elegans (CeSMOC-1) 中唯一的SMOC蛋白的功能.
- 研究CeSMOC-1在调节BMP信号传递中的分子机制.
- 确定CeSMOC-1,LON-2/glypican和DBL-1/BMP之间的相互作用.
主要方法:
- 生物化学测定 生物化学测定
- 结构建模 结构建模
- 分子遗传学 分子遗传学
- 在体外和体内活体功能研究.
主要成果:
- CeSMOC-1通过其EC域与GPC3同类LON-2/glypican结合.
- CeSMOC-1 结合到 BMP2/4 同源 DBL-1 的成熟域.
- CeSMOC-1以LON-2/glypican依赖的方式作为负调节剂.
- CeSMOC-1以DBL-1/BMP依赖的方式作为一个积极的调节器.
- 在分析表明SMOC蛋白质结合成熟的BMP二次体.
结论:
- CeSMOC-1在调节BMP信号方面具有双重作用.
- CeSMOC-1的EC域对LON-2/glypican相互作用至关重要.
- 对于 DBL-1 / BMP 相互作用,需要全长的 CeSMOC-1.
- 在BMP法规中为保存的SMOC蛋白功能提供了机制基础.
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