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相关实验视频

Updated: Jul 19, 2025

Author Spotlight: Advancing Optogenetics Research Using Lustro
03:26

Author Spotlight: Advancing Optogenetics Research Using Lustro

Published on: August 4, 2023

730

在酵母中进行高通量光遗传学实验,使用自动化平台Lustro.

Zachary P Harmer1, Megan N McClean2

  • 1Department of Biomedical Engineering, University of Wisconsin-Madison.

Journal of visualized experiments : JoVE
|August 17, 2023
PubMed
概括

我们开发了Lustro,这是一个用于光遗传系统高通量选的自动化平台. 该系统结合了光刺激和实验室自动化,以加速光遗传学工具的优化,以控制基因表达.

科学领域:

  • 合成生物学 合成生物学
  • 生物技术是生物技术.
  • 分子生物学分子生物学

背景情况:

  • 光遗传学可以通过光敏感蛋白来精确控制细胞功能.
  • 优化光遗传系统通常涉及劳累和耗时的设计-构建-测试周期.
  • 需要高通量选方法来加快新型光遗传工具的开发.

研究的目的:

  • 介绍Lustro,一个集实验室自动化和光刺激的新平台,用于高效的光遗传系统表征.
  • 为利用Lustro在光遗传系统的高通量选中提供详细的协议.
  • 为了证明Lustro在Saccharomyces cerevisiae中优化基因表达控制的应用.

主要方法:

  • 卢斯特罗采用了一台自动化工作站,配备了集成的照明,震动和盘子阅读设备.
  • 一个机器人手臂自动化了微波板的运动,用于连续刺激和测量设备之间的设备.
  • 该协议详细介绍了用于自动化实验工作流程的Lustro的设置,组件集成和编程.

主要成果:

  • 卢斯特罗能够有效地进行高通量选和光遗传系统的表征.
  • 自动化工作流程减少了光遗传系统优化所需的时间和劳动力.
  • 该平台为评估光遗传系统性能提供了精确的数据采集.

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Design and Implementation of an Automated Illuminating, Culturing, and Sampling System for Microbial Optogenetic Applications
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Light-Controlled Fermentations for Microbial Chemical and Protein Production

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Last Updated: Jul 19, 2025

Author Spotlight: Advancing Optogenetics Research Using Lustro
03:26

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Published on: August 4, 2023

730
Design and Implementation of an Automated Illuminating, Culturing, and Sampling System for Microbial Optogenetic Applications
11:13

Design and Implementation of an Automated Illuminating, Culturing, and Sampling System for Microbial Optogenetic Applications

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结论:

  • 卢斯特罗显著提高了开发和优化光遗传工具的效率.
  • 像Lustro这样的自动化平台对于推进合成生物学应用至关重要.
  • 这一协议是研究人员在酵母中研究光遗传学的宝贵资源.