相关实验视频

Updated: Jul 19, 2025

A Mouse Model of Orthopedic Surgery to Study Postoperative Cognitive Dysfunction and Tissue Regeneration

A Mouse Model of Orthopedic Surgery to Study Postoperative Cognitive Dysfunction and Tissue Regeneration

Published on: February 27, 2018

通过抑制神经炎症和氧化应激,LXA4可以减轻外科期间的神经认知障碍

Sufang Jiang¹, Qian Wan¹, Xueji Wang¹

¹Department of Anesthesiology, The Second Hospital of Hebei Medical University, No 215 Heping West Road, Shijiazhuang, Hebei, China.

International immunopharmacology

|August 17, 2023

概括

素A4 (LXA4) 通过降低神经炎症和微质激活来防止手术诱导的认知衰退. 这项研究表明,LXA4可能是围手术期神经认知障碍 (PND) 的治疗剂.

科学领域:

神经科学是一个神经科学.
免疫学免疫学免疫学
药理学药理学是指药理学的学科.

背景情况:

经手术的神经认知障碍 (PND) 是老年手术患者的常见并发症,与神经炎症和微质激活有关.
脂质媒介体利素A4 (LXA4) 具有已知的抗炎性质.
了解LXA4在PND中的作用对于开发有效的治疗方法至关重要.

研究的目的:

研究LXA4对手术引起的认知缺陷的保护作用.
探索LXA4在神经炎症和微质激活的背景下作用的潜在机制.

主要方法:

建立了PND的动物模型,使用在sevoflurane麻醉下进行腹腔切除的小鼠.
在手术前,LXA4被输入腹膜内.
使用BV2微质细胞来研究LXA4对M1/M2极化,细胞因子产生,氧化应激标志物和SIRT1/NF-κB通路的影响.

主要成果:

在PND模型中,LXA4显著缓解认知障碍,减少海马神经炎症和微质激活.
在体外,LXA4抑制了M1微质极化,促进了M2极化,并调节了亲和抗炎细胞因子水平.
LXA4减弱了氧化应激标志物 (ROS,NOX2,HO-1) 和增强了抗氧化活性 (SOD1,SOD),由SIRT1激活和NF-κB抑制介导的效果.

结论:

LXA4对手术诱导的认知缺陷和神经炎症表现出显著的神经保护作用.
在PND中LXA4的治疗潜力很可能通过微质中的SIRT1/NF-κB信号通路进行介导.
LXA4是缓解神经炎症,氧化应激和与PND相关的认知功能障碍的有希望的治疗候选者.

关键词:

在LXA4中使用.微质细胞中的微质细胞神经炎症是一种神经炎症.氧化应激是一种氧化应激.在外科手术期间出现的神经认知障碍.一个SIRT1系统.

更多相关视频

Author Spotlight: Improving Anesthesia Protocols for Enhanced Mouse Acupuncture Research

Author Spotlight: Improving Anesthesia Protocols for Enhanced Mouse Acupuncture Research

Published on: December 8, 2023

Lateral Chronic Cranial Window Preparation Enables In Vivo Observation Following Distal Middle Cerebral Artery Occlusion in Mice

Lateral Chronic Cranial Window Preparation Enables In Vivo Observation Following Distal Middle Cerebral Artery Occlusion in Mice

Published on: December 29, 2016

相关概念视频

Local Anesthetics: Pharmacokinetics

Local Anesthetics: Pharmacokinetics

The potency and duration of action of local anesthetics (LAs) are determined by their pharmacokinetics. Pharmacokinetics describes how LAs are absorbed, distributed, metabolized, and eliminated from the body. When administered to the vascular tissues, LAs are quickly absorbed and enter the systemic circulation, reducing their localized effects. Adding vasoconstrictors such as epinephrine to LAs reduces their absorption into the systemic circulation, making them clinically effective. The...

Local Anesthetics: Mechanism of Action

Local Anesthetics: Mechanism of Action

Local anesthetics (LAs) block sensory and motor impulses by inhibiting the sodium channels on the nerve cell membranes. This induces temporary loss of sensation, relieving pain in a specific body area.
Local anesthetics are amphiphilic molecules consisting of a hydrophobic aromatic part linked to a hydrophilic group by an ester or amide linkage. They are weak bases and are usually available as salts, which increases their solubility and stability. Once administered, LAs exist in the body either...

Local Anesthetics: Clinical Application as Spinal Anesthesia

Local Anesthetics: Clinical Application as Spinal Anesthesia

Spinal anesthetics are given during lower abdomen and limb surgeries to block sensory and motor neurons. They are administered in the mid to low lumbar regions, primarily acting on the cauda equina's nerve roots. The blockade level depends on the local anesthetic (LA) concentration. Usually, low LA concentrations are sufficient to block sensory fibers, while only high LA concentrations block motor fibers. Other factors like injection volume and speed, the patient's posture, and the drug...

Local Anesthetics: Clinical Application as Epidural Anesthesia

Local Anesthetics: Clinical Application as Epidural Anesthesia

Epidural anesthetics are administered in the fat-filled epidural space, the outermost part of the spinal canal. This technique is commonly employed for pain management and anesthesia during lower abdomen and pelvis surgeries or labor and delivery.
Since epidural anesthetics can be infused through an epidural catheter, all types of drugs, including short-acting ones, can be administered. Chloroprocaine and lidocaine are examples of short and long-duration anesthetics, respectively. Bupivacaine...

Local Anesthetics: Adverse Effects

Local Anesthetics: Adverse Effects

While local anesthetics are generally safe and well-tolerated, they can occasionally cause adverse effects that vary in severity. Local anesthetics can induce toxicity at two distinct levels. They can either produce local effects through direct contact with the neural elements or be absorbed into the bloodstream from the injection site, leading to systemic effects.
Once absorbed into the systemic circulation, local anesthetics can affect the organs that depend on the functioning of sodium...

Local Anesthetics: Differential Sensitivity of Nerve Fibers

Local Anesthetics: Differential Sensitivity of Nerve Fibers

Local anesthetics (LAs) block the sodium channels of nerve trunks, sensory nerve endings, and neuromuscular junctions. Although LAs can block all kinds of nerves, the sensitivity of nerve fibers differs according to nerve types and structures. LAs are known to block myelinated fibers faster than unmyelinated ones. Also, they block pain or sensory neurons at low concentrations without affecting the motor neurons involved in muscle contractions. This helps relieve labor pain without affecting the...